About Enhancer
| Enhancer ID: | E_02_309 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr12:113302447-113305102   |
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| Biosample name: | Blood | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 26883548 |
| Enhancer experiment: | PCR,ChIP | |
| Enhancer experiment description: | Extracted DNA was amplified by PCR and submitted to high_x0002_throughput sequencing to evaluate SHM. As SHM in light chains is not under the 3’RR control9, IgH SHM values along rearranged JH4 sequences were normalised to Jk5 SHM values. Mutation frequencies of 1.45% and 0.07% were found in wt and AID _x0002_ -/-_x0002_mice, respectively. SHM frequency was markedly reduced (by more than fourfold, at 0.33%) in DleftPAL mice (Fig. 2a,b).The presence of hs3a and hs1,2 enhancers in DIRIS mice maintained SHM frequency at 0.75%, that is, at an intermediate level higher than DleftPAL mice (Fig. 2a,b) but markedly lower(by about twofold) than in wt mice (Fig. 2a,b). |
About Target gene
| Target gene : | Ighg3(IgG3) | |
| Strong evidence: | -- | |
| Less strong evidence: | RNA-seq,ChIP | |
| Target gene experiment description: | RNA-seq experiments showed that Cg3 and Cg2b transcription(Fig. 5a and d, respectively) was markedly reduced in DleftPAL mice compared with wt mice. The presence of hs3a and hs1,2 enhancers in DIRIS mice preserved Cg3 and Cg2b transcription.Levels of H3K4me3 (Fig. 5b,e) and H3K27ac (Fig. 5c,f) in the Ig3- S g3-C g3 and Ig2b-S g2b-C g2b regions (during IgG3 and IgG2b CSR,respectively) were reduced only on deletion of 3’RR enhancers but preserved in DIRIS mice. |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | This shows a synergistic role of enhancers and of the palindromic architecture This shows a synergistic role of enhancers and of the palindromic architecture for induction of epigenetic modifications and chromatin accessibility. |
| Enhancer function experiment: | ChIP |
| Enhancer function experiment description: |
In conclusion, this work indicates that the IgH 3'RR does not simply pile up enhancer units but also optimally exposes them into a functional architecture of crucial importance. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
