About Enhancer
| Enhancer ID: | E_02_308 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr4:115040426-115042426   |
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| Biosample name: | HPC-7 | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | Malignant Hematopoiesis | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | -- | |
| Pubmed ID: | 26809507 |
| Enhancer experiment: | DNaseI-seq,ChIP,ChIP-seq,Hi-C,Transgenic mice | |
| Enhancer experiment description: | Identification of putative enhancers based on ChIP-Seq data alone cannot assign distal regions to specific genes with confidence because enhancers are known.to have the ability to act over large distances, and may loop over intervening genes.39 To overcome this limitation, we made use of our CHi-C interaction list, and filtered our list of putative enhancers to only retain those that looped to the promoter regions of known regulators of HSPC function. |
About Target gene
| Target gene : | Scl | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP-seq,Hi-C | |
| Target gene experiment description: | Visualization of the interaction files together with our previously published 10 TF ChIP-Seq demonstrated specific interactions of the Scl (also known as Tal1) and Lmo2 promoters with the previously characterized enhancer regions at Scl -15 kb,+19 kb, and +40 kb, as well as Lmo2 -75 kb,-70 kb,-64 kb, and the proximal promoter (pPex). |
About TF
| TF name : | Cebpa(C/EBP-alpha,CEBP)Cebpb(C/EBP-beta,IL6DBP,NF-IL6,TCF5)Fos(AP-1,C-FOS, p55)Myc(MRTLC,bHLHe39,c-Myc,MYC)E2f4(2010111M04Rik,AI427446)Egr1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225)Elf1(EFTUD1,RIA1)Cbfa2t3Jun(AP-1,AP1,c-Jun,cJUN,p39)Ldb1MaxMyb(Cmyb,c-myb,c-myb_CDS,efg)Nfe2(NF-E2,p45)Trp53Rad21Stat3(ADMIO,ADMIO1,APRF,HIES) | |
| TF experiment: | DNaseI-seq,ChIP-seq,Hi-C | |
| TF experiment description: | Given that HPC-7 represents one of the best in vitro models for HSPCs, we wanted to bring genomic information for these cells up to a similar level of completeness, and therefore performed ChIP-Seq experiments for a further 17 TFs (CEBPa,CEBPb, cFOS, cMYC, E2F4, EGR1, ELF1, ETO2,c-JUN,LDB1,MAX, MYB, NFE2, p53, RAD21, pSTAT1, and STAT3) as well as genome-wide DNase I hypersensitive mapping and 3 additional his_x0002_tone marks (H2AK5ac, H3K4me3, and H3K36me3). |
About Function
| Enhancer function : | Scl Enhancer elements also interact with the promoter of the neighboring Pdzk1ip1 gene, consistent with previous reports suggesting that Scl and Pdzk1ip4 form a single transcriptional domain. |
| Enhancer function experiment: | Hi-C,ChIP-seq |
| Enhancer function experiment description: |
Analysis of well_x0002_characterized gene loci encoding key HSPC regulators therefore suggests that the newly generated CHi-C data set represents a valuable resource to advance our understanding of transcriptional control mechanisms in HSPCs. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
