About Enhancer
| Enhancer ID: | E_02_296 | |
| Enhancer symbol: | Ed | |
| Species: | Mouse | |
| Position : | chr6:67560136-67562136   |
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| Biosample name: | B Cell,T Cell,MPC-11 | |
| Experiment class : | Low throughput |
| Enhancer type: | enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 15798207 |
| Enhancer experiment: | 3C,PCR | |
| Enhancer experiment description: | The mouse immunoglobulin kappa (Igk) gene contains an intronic enhancer and two enhancers downstream of its transcription unit. Using chromosome conformation capture technology, we demonstrate that rearranged and actively transcribed Igk alleles in MPC-11 plasmacytoma cells exhibit mutual interactions over 22 kb between these three enhancers and Vk gene promoters. We also observe interactions between Ei and E3'with 3'boundary sequences 24 kb downstream of Ed, adjacent to a neighboring house_x0002_keeping gene. |
About Target gene
| Target gene : | Igk(kappa) | |
| Strong evidence: | 3C | |
| Less strong evidence: | ChIP,Southern blot | |
| Target gene experiment description: | Chromatin segments within the transcription unit exhibit interactions throughout regions downstream of the tran_x0002_scription termination region specifically in active Igk loci. |
About TF
| TF name : | Rela(p65,p65 NF-kappa B,p65 NFkB)Tcf3(A1,AA408400,ALF2,AW209082,E12,E12/E47,E2A,E47,KA1,ME2,Pan1,Pan2,TCF-3,Tcfe2a,VDIR,bHLHb21)Tfap4 | |
| TF experiment: | ChIP,PCR | |
| TF experiment description: | Taken together with the hypothesis that proteins that may be recruited by NF-kB and/or E2A might exhibit mutual bridging interactions between these transcription factors on different cis elements, we decided to first assay for the presence of NF-kB and E2A on the Igk gene enhancers and the Vk19-17 gene by performing ChIP experiments. |
About Function
| Enhancer function : | We observe interactions between Ei and E3' with 3' boundary sequences 24 kb downstream of Ed, adjacent to a neighboring house-keeping gene. |
| Enhancer function experiment: | 3C |
| Enhancer function experiment description: |
These results fit a looping mechanism for enhancer function like in the beta-globin locus and suggest a dynamic modulation of the spatial organization of the active Igkappa locus. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
