Enhancer ID: | E_02_292 | |
Enhancer symbol: | ||
Species: | Mouse | |
Position : | chr12:103853032-103853127 |
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Biosample name: | Hepatoma Cells | |
Experiment class : | low throughput |
Enhancer type: | enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | -- | |
Pubmed ID: | 2835657 |
Enhancer experiment: | Transient Assay,EMSA | |
Enhancer experiment description: | To test whether the more distant upstream sequences could function as an enhancer, the -523- to -168-bp restriction fragment was attached in either orientation (at -341 bp)to a mouse ,beta-globin promoter.The results of transfection experiments showed a 10- to 15-fold enhancement of beta-globin transcription when this region was present in either orientation . |
Target gene : | Serpina1a | |
Strong evidence: | -- | |
Less strong evidence: | Transient Assay,EMSA | |
Target gene experiment description: | Proteins present in nuclear extracts bind to the functionally defined elements present within the al-AT enhancer. Nuclear extracts from Hep-G2 cells were incubated with end-labeled DNA probes from the enhancer region to detect nuclear protein-binding sites by the gel retardation or gel shift assay. Each protein-binding site correlated with a functionally importantregion necessary for full enhancer activity. These experiments demonstrated a complex arrangement of regulatory elements comprising the al-antitrypsin enhancer. |
TF name : | -- | |
TF experiment: | -- | |
TF experiment description: | -- |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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