About Enhancer
| Enhancer ID: | E_02_283 | |
| Enhancer symbol: | miR-148a SE2 | |
| Species: | Mouse | |
| Position : | chr6:51168034-51174811   |
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| Biosample name: | Pro-B cell | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | -- | |
| Pubmed ID: | 26855180 |
| Enhancer experiment: | CRISPR/Cas9,RT-qPCR | |
| Enhancer experiment description: | We performed a functional dissection ofmiRNA SEs in vivo by generating cell lines depleted of individual miRNA SE constituents (about 400–700 bp) in multiple cell types through a CRISPR/Cas9-based approach (Table S5). We analyzed three miRNA SEs: miR-290-295 SE in mESCs, miR-1 SE in myotubes, and miR-148a SE in Pro-B cells.Furthermore, in Pro-B cells, deletion of four out of five miR-148a SE constituents dynamically suppressed induction of miR-148a during differenti- ation and affected its downstream genes Bach2 and Blimp1(Porstner et al., 2015)(Figures 4E and 4F). Interestingly, in Pro-Bcells,the effects of deletions of SE constituents on pri-miRNAs were minor, but the reductions in pri-miRNA processing were more pronounced (Figure 4I). |
About Target gene
| Target gene : | MiR-148a(Mirn148,Mirn148a,mir-148a) | |
| Strong evidence: | CRISPR/Cas9 | |
| Less strong evidence: | RT-qPCR | |
| Target gene experiment description: | We performed a functional dissection ofmiRNA SEs in vivo by generating cell lines depleted of individual miRNA SE constituents (about 400–700 bp) in multiple cell types through a CRISPR/Cas9-based approach (Table S5). We analyzed three miRNA SEs: miR-290-295 SE in mESCs, miR-1 SE in myotubes, and miR-148a SE in Pro-B cells.Furthermore, in Pro-B cells, deletion of four out of five miR-148a SE constituents dynamically suppressed induction of miR-148a during differenti- ation and affected its downstream genes Bach2 and Blimp1(Porstner et al., 2015)(Figures 4E and 4F). Interestingly, in Pro-Bcells,the effects of deletions of SE constituents on pri-miRNAs were minor, but the reductions in pri-miRNA processing were more pronounced (Figure 4I). |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
