About Enhancer
| Enhancer ID: | E_02_269 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr6:52527210-52556565   |
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| Biosample name: | Limb,Head | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | Limb malformation | |
| DO: | -- | |
| Mesh: | D017880 | |
| Distance from TSS: | <2KB | |
| Pubmed ID: | 24385922 |
| Enhancer experiment: | ChIP-seq,3C,5C | |
| Enhancer experiment description: | Active enhancers are characterized by the binding of several proteins including RNA polymerase II (RNAP2), and subunits of Mediator like Med12.We therefore mapped candidate enhancer sequences by identifying genomic sites enriched in these proteins using chromatin immunoprecipitation combined with deep sequencing(ChIP-seq) in cells isolated from E12.5 distal limb buds.Candidate HoxA enhancers therefore reside amidst other genes including Hibadh, Tax1bp1, and Jazf1, for which expression has been reported in the limb.Consistent with our 3C data, this 5C analysis revealed a similar contact pattern between the 59 HoxA genes and upstream regulatory region in the Shh2/2 mutant and wt limbs. |
About Target gene
| Target gene : | Hoxa(Hox-1) | |
| Strong evidence: | 5C,3C | |
| Less strong evidence: | -- | |
| Target gene experiment description: | We profiled the interaction pattern of the HoxA cluster with the upstream 850 Kb region in distal limb buds and head tissues using 5C technology combined with deep sequencing.We found that the 59 part of HoxA, containing Hoxa9 to Hoxa13, frequently interacts with several regions upstream of the cluster,and that most of these regions contain the candidate limb enhancers. |
About TF
| TF name : | Ctcf(MRD21) | |
| TF experiment: | 5C | |
| TF experiment description: | Interestingly,loci bound either by CTCF or cohesin in limb buds have been recently identified and comparison with our data shows that almost all loci interacting with 59 HoxA genes overlap with either CTCF cohesin binding. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
