About Enhancer
| Enhancer ID: | E_02_266 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr14:67745323-67746327   |
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| Biosample name: | GT1-1 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | <2KB | |
| Pubmed ID: | 21527504 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP | |
| Enhancer experiment description: | To examine the enhancer activity of intron A, we constructed reporter vectors containing the full-length mouse intron A sequence (1003 bp) in either the forward or reversed direction followed by the simian vacuolating virus 40 (SV40) minimal promoter (SV40p) driving luciferase expression.These findings strongly suggest that GnRH intron A contains a putative transcriptional enhancer that is likely involved in GnRH gene regulation. In an ensuing experiment, serially deleted and truncated reporter constructs revealed that 315 nucleotides (nt) of GnRH intron A, located proximal to Ex1, were responsible for its transcription enhancing activity; the enhancing activity of this region was comparable with that of the full-length intron A. |
About Target gene
| Target gene : | Gnrh1(Gnrh,Gnrh2,LHRH,Lhrh1,Lnrh,hpg) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP,RT-qPCR | |
| Target gene experiment description: | GnRH is a pivotal hypothalamic neurohormone governing reproduction and sexual development. Because transcriptional regulation is crucial for the spatial and temporal expression of the GnRH gene, a region approximately 3.0 kb upstream of the mammalian GnRH promoter has been extensive studied. In the present study, we demonstrate a transcription-enhancer located in the first intron (intron A) region of the GnRH gene. |
About TF
| TF name : | Qsox1(1300003H02Rik,QSOX,Qscn6,SOx,b2b2673Clo) | |
| TF experiment: | ChIP,EMSA | |
| TF experiment description: | Chromatin immunoprecipitation (ChIP) assay revealed that SOX11 binds to the Ex1-proximal Enhancer region of intron A, and overexpression of SOX11 in creases this binding in GT1-1 cells. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
