Enhancer ID: | E_02_251 | |
Enhancer symbol: | Ciita Enhancer | |
Species: | Mouse | |
Position : | chr16:10440279-10442279 |
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Biosample name: | Macrophage | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 30327417 |
Enhancer experiment: | qChIP | |
Enhancer experiment description: | We then analyzed peak A and Ciita promoter I by qChIP in NFAT5-deficient or control macrophages for different histone modifications that are enriched in gene enhancers (H3K4me1) or promoters (H3K4me3) and a modification that marks transcribed regions and active enhancers.We observed that peak A region had a slightly higher content in H3K4me1 compared with Ciita promoter I, and this was NFAT5 independent. |
Target gene : | Ciita(C2ta,EG669998,Gm9475,Mhc2ta) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP-seq | |
Target gene experiment description: | ChIP-seq analysis of wild-type and NFAT5-deficient BMDMs showing the position of NFAT5-binding site peak A 47 kb upstream of the Ciita locus. |
TF name : | Nfat5(AI225870,B130038B15Rik,CAG-8,CAG80,NFATL1,OREBP,TonEBP,mKIAA0827,nfatz) | |
TF experiment: | qChIP | |
TF experiment description: | We therefore asked whether NFAT5 bound these regulatory regions. Quantitative chromatin immunoprecipitation (qChIP) with a combination of two polyclonal antibodies to NFAT5 detected its specific binding to promoter I of Ciita and the promoter of H2-Ab both in untreated macrophages as well as in macrophages treated with IFNγ.This result indicated that NFAT5 is a relevant factor to control Ciita expression in macrophages through its myeloid promoter I. Altogether,our analysis of primary myeloid and lymphoid APCs uncovered a selective requirement of NFAT5 in macrophages for expressing CIITA and MHCII. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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