About Enhancer
| Enhancer ID: | E_02_232 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr6:127033997-127036097   |
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| Biosample name: | Osteocyte Cell Lines | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | Chronic Kidney Disease | |
| DO: | DOID:784 | |
| Mesh: | D051436 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 29527594 |
| Enhancer experiment: | ChIP-seq,CRISPR/Cas9 | |
| Enhancer experiment description: | We therefore utilized chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) data from an osteocyte cell line to identify potential regulatory regions of the Fgf23 gene. Based on ChIP-seq analysis of enhancer_x0002_associated histone modifications, including H3K4 methylation and H3K9 acetylation, we discovered several potential enhancers for Fgf23, one of which was located 16kb upstream of the gene’s transcriptional start site. |
About Target gene
| Target gene : | Fgf23(Fgf23) | |
| Strong evidence: | CRISPR/Cas9 | |
| Less strong evidence: | ChIP-seq,RT-PCR | |
| Target gene experiment description: | Nevertheless, lack of the _x0003_16kb enhancer blunted FGF23 upregulation in a tissue-specific manner by the acute inflammatory inducers lipopolysaccharide (LPS), interleukin-1-beta (IL-1b), and tumor necrosis factor-alpha (TNFa) in bone, non-osseous tissues,and in circulation. Lack of the _x0003_16kb enhancer also inhibited PTH-induced bone Fgf23 mRNA. Moreover, the absence of this Fgf23 enhancer in an oxalate diet-induced murine CKD model prevented the early onset induction of osseous, renal, and thymic Fgf23 mRNA levels and led to a significant blunting of elevated circulating intact FGF23 levels. These results suggest that _x0003_16kb enhancer mediates the induction of Fgf23 by inflammation and PTH and facilitates the increase in FGF23 expression in a murine model of CKD. |
About TF
| TF name : | Nfkb1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50)Hif1a(HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78) | |
| TF experiment: | ChIP-seq | |
| TF experiment description: | Advances in genome‐wide techniques such as chromatin immunoprecipitation followed by DNA sequencing (ChIP‐seq) have revealed that the transcriptional regulation of many genes, including receptor activator of NF‐κB ligand (Tnfsf11), vitamin D receptor (Vdr), or matrix metallopeptidase 13 (Mmp13), are mediated through a complex network of Enhancers each providing distinct factor‐ and cell‐type specificity. |
About Function
| Enhancer function : | 16kb Enhancer mediates the induction of Fgf23 by inflammation and PTH and facilitates the increase in FGF23 expression in a murine model of CKD |
| Enhancer function experiment: | ChIP-seq |
| Enhancer function experiment description: |
These results suggest that 16kb Enhancer mediates the induction of Fgf23 by inflammation and PTH and facilitates the increase in FGF23 expression in a murine model of CKD. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
