About Enhancer
| Enhancer ID: | E_02_225 | |
| Enhancer symbol: | MdE Enhancer | |
| Species: | Mouse | |
| Position : | chr1:133347574-133364574   |
|
| Biosample name: | As4.1,Tsukuba hypertensive mice | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Hypertension | |
| DO: | DOID:10763 | |
| Mesh: | D006973 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 29358217 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP | |
| Enhancer experiment description: | Because the 17-kb sequence exhibited Enhancer activity in vivo and in vitro,we narrowed down the Enhancer to a 2.3-kb core using Luciferase assays in As4.1 cells. When this 2.3-kb sequence was removed from the endogenous mRen gene in the mouse,its basal expression was dramatically reduced,and the hypertension esponsiveness was significantly attenuated. |
About Target gene
| Target gene : | Ren1(D19352,Ren,Ren-1,Ren-Ac,Ren1d,Rn-1,Rnr,Ren1) | |
| Strong evidence: | CRISPR/Cas9 | |
| Less strong evidence: | qRT-PCR,ChIP | |
| Target gene experiment description: | While the mRen gene bearing the 3' deletion was appropriately downregulated, the one bearing the 5'deletion lost this hypertension responsiveness. Because the 17-kb sequence exhib_x0002_ited enhancer activity in vivo and in vitro, we narrowed down the enhancer to a 2.3-kb core using luciferase assays in As4.1 cells. When this 2.3-kb sequence was re_x0002_moved from the endogenous mRen gene in the mouse, its basal expression was dra_x0002_matically reduced, and the hypertension responsiveness was significantly attenuated. |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | This finding sheds light on the unexplored distal regulatory region of the mRen gene and provides in vivo evidence that a novel 5' enhancer plays an essential role in the physiological response to an AII-induced hypertensive environment. |
| Enhancer function experiment: | qRT-PCR |
| Enhancer function experiment description: |
We propose that in a hypertensive environment, the activity of this novel enhancer is attenuated, and, as a consequence, mRen gene transcription is suppressed to maintain blood pressure. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
