About Enhancer
| Enhancer ID: | E_02_223 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr7:143462624-143463585   |
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| Biosample name: | Mouse Embryonic Fibroblasts | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Malignancies | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 29284139 |
| Enhancer experiment: | Luciferase Reporter Assay | |
| Enhancer experiment description: | As shown in Fig 5A, enh1 is in the second intron of Cdkn1c while enh2 is located in the 3’ UTR. Luciferase reporter constructs were transfected into iMEFs stably overexpressing FOXD1 or a negative control vector. |
About Target gene
| Target gene : | Cdkn1c(AL024410,CDKI,Kip2,p57(kip2),p57Kip2) | |
| Strong evidence: | CRISPR/Cas9 | |
| Less strong evidence: | Luciferase Reporter Assay,RT-PCR | |
| Target gene experiment description: | We tested whether FOXD1 directly binds these enhancer sequences using established luciferase reporter constructs. As shown in Fig. 5A, enh1 is in the second intron of Cdkn1c while enh2 is located in the 3′ UTR. Luciferase reporter constructs were transfected into iMEFs stably overexpressing FOXD1 or a negative control vector. FOXD1-expressing cells exhibited higher activity of enh1-driven luciferase than negative controls while no difference was observed in luciferase activity driven by enh2(Fig. 5B). |
About TF
| TF name : | Gli1(AV235269,Zfp-5,Zfp5) | |
| TF experiment: | RT-PCR,CRISPR/Cas9 | |
| TF experiment description: | SHH-regulation of Ccnd1 and Cdkn1c also appeared dependent upon the GLI transcription factors, as SHH ligand addition in Gli2 iMEFs did not change their expression. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
