Enhancer ID: | E_02_222 | |
Enhancer symbol: | -- | |
Species: | Mouse | |
Position : | chr7:143459172-143459787 |
|
Biosample name: | Mouse Embryonic Fibroblasts | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | Malignancies | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | <2KB | |
Pubmed ID: | 29284139 |
Enhancer experiment: | Luciferase Reporter Assay | |
Enhancer experiment description: | As shown in Fig 5A, enh1 is in the second intron of Cdkn1c while enh2 is located in the 3’ UTR. Luciferase reporter constructs were transfected into iMEFs stably overexpressing FOXD1 or a negative control vector. |
Target gene : | Cdkn1c(AL024410,CDKI,Kip2,p57(kip2),p57Kip2) | |
Strong evidence: | CRISPR/Cas9 | |
Less strong evidence: | Luciferase Reporter Assay,RT-PCR | |
Target gene experiment description: | We tested whether FOXD1 directly binds these enhancer sequences using established luciferase reporter constructs. As shown in Fig. 5A, enh1 is in the second intron of Cdkn1c while enh2 is located in the 3′ UTR. Luciferase reporter constructs were transfected into iMEFs stably overexpressing FOXD1 or a negative control vector. FOXD1-expressing cells exhibited higher activity of enh1-driven luciferase than negative controls while no difference was observed in luciferase activity driven by enh2(Fig. 5B). |
TF name : | Foxd1(AI385632,BF-2,FREAC4,Hfh10,Hfhbf2) | |
TF experiment: | Luciferase Reporter Assay | |
TF experiment description: | Firefly luciferase reporter assay driven by Cdkn1c enhancer elements, enh1 and enh2, in iMEFs overexpressing empty pLenti construct or FOXD1. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
-- |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
Home | Browse | Search | Download | Genome-Browser | Submit | Contact | Help
Copyright © HMU | 黑ICP备16009434号-1 | Li C Lab