About Enhancer
| Enhancer ID: | E_02_203 | |
| Enhancer symbol: | Il2ra Enhancer | |
| Species: | Mouse | |
| Position : | chr8:11564419-11614821   |
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| Biosample name: | T Cell | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 29078395 |
| Enhancer experiment: | ChIP-seq,ChIA-PET | |
| Enhancer experiment description: | To clarify how distal enhancer elements within superenhancer loci form enhancer-promoter loops and chromatin interactions, we performed RNA Pol II-based chromatin interaction analysis by paired-end tag (ChIA-PET) sequencing (24, 25) using mouse CD4+ T cells that were preactivated by TCR stimulation (anti-CD3 plus anti-CD28) and then not treated or treated with IL-2 for 4 h.Interestingly, both H3K4me3 (blue peaks in Fig. 3, primarily at the promoter) and H3K27Ac (green peaks in Fig. 3, spanning the Il2ra superenhancer region; also shown in Fig. 1E) were strong after TCR preactivation and only modestly affected by stimulation with IL-2, in marked contrast to the changes in STAT5 binding and chromatin looping. |
About Target gene
| Target gene : | Il2ra(CD25,IDDM10,IL2R,IMD41,TCGFR,p55) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP-seq,RNA-seq | |
| Target gene experiment description: | As noted above, STAT5 and H3K27Ac are highly enriched at a subset of superenhancers, including at the Il2ra superenhancer (Fig. 1 A, B, and E), correlating with IL-2–induced gene expression (Fig. 2B and Fig. S1C). We also found extensive H3K27Ac and STAT5 binding at the human IL2RA locus (Fig. S2), analogous to the mouse Il2ra superenhancer, with significant conservation of some of the enhancer elements (Fig. S2). |
About TF
| TF name : | Stat5a(AA959963,STAT5)Stat3(ADMIO,ADMIO1,APRF,HIES) | |
| TF experiment: | ChIP-seq,CRISPR/Cas9 | |
| TF experiment description: | Based on our genome-wide analysis of chromatin immunoprecipitation coupled to DNA sequencing (ChIP-Seq) profiles, we observed that STAT5-enriched Super-Enhancers spanned large genomic regions (average size of ～19 kb) and indeed were associated with high levels of H3K27Ac. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
