About Enhancer
| Enhancer ID: | E_02_193 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr1:136170878-136192613   |
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| Biosample name: | C2C12 | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 28575289 |
| Enhancer experiment: | ChIP-seq | |
| Enhancer experiment description: | To further monitor the progressive remodeling of SE during the MB differentiation into MTs, we generated a time series of H3K27ac ChIP-seq data covering the differentiating course of −24,0,6,12,24 and 72 hr (Supplementary Data S2). As shown in Figure Figure1R,1R, progressive temporal changes in enhancer usage were readily observed during the progression of differentiation. |
About Target gene
| Target gene : | Myog(MYF4,bHLHc3,myo) | |
| Strong evidence: | 3C | |
| Less strong evidence: | qRT-PCR,ChIP-seq | |
| Target gene experiment description: | To further illuminate whether the cooperation is mediated through physical interaction through chromosomal looping, 3C (chromosome confirmation capture) assay was performed followed by PCR to analyze the chromatin loop structure (Figure (Figure5L).5L). Detectable looping frequency between H3 and H4 or the promoter but not with the negative control regions upstream of H1 or downstream of Myogenin gene was observed upon early differentiation at 24 h and persisted throughout the differentiation whereas the interaction between H3 and H1 or H2 occurred in a later stage (Figure (Figure5L5L–N and Supplementary Figure S6F), confirming H3 is the earliest to be activated then probably helped the establishment of H4 and others through their interactions. |
About TF
| TF name : | Foxo3(1110048B16Rik,2010203A17Rik,C76856,FKHRL1,Fkhr2a,Foxo3)Myod1(AI503393,MYF3,MyoD,Myod-1,bHLHc1) | |
| TF experiment: | CRISPR/Cas9,Western blot | |
| TF experiment description: | Indeed, the deletion of MyoD by CRISPR-Cas9 in the cells (Supplementary Figure S3A–D) induced extraordinary changes at SE landscape; 584 SEs in WT were lost while 257 new ones were gained (Supplementary Figure S3E and F). The deletion not only sharply attenuated the enhancer activity at the WT SE hotspots (Figure (Figure3A),3A), but also resulted in the activation of genes that acquired de novo SEs in KO cells (Supplementary Figure S3G).HA-tagged MyoD was co-transfected with plasmid expressing Flag-tagged full length FoxO3. Lysates were pulled down by HA antibody and examined by western blotting using Flag antibody. For all the above panels, the representative images from two replicates are shown. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
