About Enhancer
| Enhancer ID: | E_02_143 | |
| Enhancer symbol: | Ccnb1ip1 Enhancer | |
| Species: | Mouse | |
| Position : | chr14:50787449-50787634   |
|
| Biosample name: | Mitotic Cell | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | <2KB | |
| Pubmed ID: | 26110280 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP,RT-PCR | |
| Enhancer experiment description: | PCR amplification of JF1 genomic DNA using several primer pairs targeted to differentially methylated regions identified a 1.8 kbp genomic segment that was present in the JF1 strain but absent in the B6 strain . Sequence analysis revealed that this 1.8kbp segment consisted of a 1,579 bp interval deleted in the B6 genome, and the remaining 220 bp interval present in the B6 genome but in an inverted orientation. |
About Target gene
| Target gene : | Ccnb1ip1(Gm288,Hei10,mei4) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay,ChIP,RT-PCR | |
| Target gene experiment description: | We hypothesized the existence of an insulator-type element that prevents the spreadof DNA methylation within the1.8 k segment,and actually identified a 242- and a 185- fragments that were located adjacent to eachother and showed insulatorand Enhancer activities,respectively,in reporter assays. We designated these genomicregions as the Ccnb1ip1 insulator and the Ccnb1ip1Enhancer. The Ccnb1ip1 insulator showed Enhancer-blocking activity in the Luciferaseiferase assays andbarrieractivity in the colony formation assays. Further examination of the Ccnb1ip1locus in other mammalian speciesrevealed that the insulator and Enhancerare High throughput lyconserve damonga wide variety of species,and are located immediately upstream of the transcriptional start site of Ccnb1ip1. |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
