Enhancer ID: | E_02_136 | |
Enhancer symbol: | -- | |
Species: | Mouse | |
Position : | chr5:119654061-119663588 |
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Biosample name: | Mouse Embryonic Stem Cell | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 25775043 |
Enhancer experiment: | CRISPR/Cas9,3C,Luciferase Reporter Assay,ATAC-seq | |
Enhancer experiment description: | We generated mouse embryonic stem cells (mESCs) expressing versions of Neisseria meningitidis (Nm) dCas9 fused with LSD2, a non-effector BirA affinity tag (BAT), or a KRAB repressor and used a viral delivery system for sgRNAs. We first targeted the well-c |
Target gene : | Tbx3(D5Ertd189e) | |
Strong evidence: | 3C,CRISPR/Cas9 | |
Less strong evidence: | PCR,ChIP | |
Target gene experiment description: | This previously unannotated ESC specific Enhancer is positioned ~10kb upstream of the transcription factor Tbx3 (Fig. 1d), a gene previously implicated in the maintenance of pluripotency.We therefore hypothesized that Enh1 may function in the ESC network by regulating Tbx3 expression. |
TF name : | Tbx3(D5Ertd189e) | |
TF experiment: | PCR,Luciferase Reporter Assay,ChIP,3C | |
TF experiment description: | This previously unannotated ESC_x0002_specific Enhancer is positioned ~10kb upstream of the transcription factor Tbx3, a gene previously implicated in the maintenance of pluripotency20. We therefore hypothesized that Enh1 may function in the ESC network by regulating Tbx3 expression. |
Enhancer function : | We conclude that the dCas9-LSD1 fusion protein allows for an effector dependent definition of functional, native enhancer elements that help to maintain a given cellular state. |
Enhancer function experiment: | 3C |
Enhancer function experiment description: |
We conclude that the dCas9-LSD1 fusion protein allows for an effector dependent definition of functional,native Enhancer elements that help to maintain a given cellular state.Accordingly,dCas9-LSD1 provides a rapid and powerful approach to understanding distal cis-regulatory regions such as Enhancers without major disruption of the local genomic architecture. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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