About Enhancer
| Enhancer ID: | E_02_132 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr8:106613868-106615868   |
|
| Biosample name: | NMuMG | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | Intron | |
| Pubmed ID: | 25652130 |
| Enhancer experiment: | Luciferase Reporter Assay,3C | |
| Enhancer experiment description: | We reported previously that intron 2 of Cdh1 is essential for proper E-cad expression.In order to analyze this large DNA sequence(>41 kb) for putative enhancers we focused on evolutionary conserved sequences. |
About Target gene
| Target gene : | Cdh1(Arc-1,BCDS1,CD324,CDHE,ECAD,LCAM,UVO) | |
| Strong evidence: | 3C | |
| Less strong evidence: | ChIP,qPCR | |
| Target gene experiment description: | Weshow that Cdh1 activity during MET is governedby two Enhancers at +7.8 and at +11.5 within intron 2 that are activated by binding of Grhl3 and Hnf4α,respectively. |
About TF
| TF name : | Hnf4a(HNF-4,Hnf4lpha,MODY1,Nr2a1,TCF-14,Tcf14,Hnf4a) | |
| TF experiment: | Luciferase Reporter Assay,ChIP | |
| TF experiment description: | Using luciferase reporter assays, we first tested whether Hnf4α could activate Cl.5 in NMuMG cells.In contrast to Grhl3 enrichment on the Cl.3 intronic sites, Hnf4α recruitment to the chromatin was detectable already in untreated NMuMG cells under steady state conditions, suggesting a distinct function for this Enhancer. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
