Enhancer ID: | E_02_130 | |
Enhancer symbol: | MyoD distal Enhancer | |
Species: | Mouse | |
Position : | chr7:46351197-46355693 |
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Biosample name: | C2C12 | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 25651906 |
Enhancer experiment: | Luciferase Reporter Assay,ChIP | |
Enhancer experiment description: | C2C12 myoblasts stably carrying the P6P (−5870 to +95) -driven reporter (MyoD-P6P-luc) or another composite reporter,MyoD-PE-luc, containing P6P plus the DE (−25277 to−20780) were treated with Wnt3a medium. We found that Wnt3a marginally, but significantly, activated MyoDP6P-luc activity; however, Wnt3a strongly activated MyoD-PE-luc, suggesting that one or multiple Wnt-response elements(WREs) are located within the DE. |
Target gene : | Myod1(MYF3,MYOD,PUM,bHLHc1) | |
Strong evidence: | -- | |
Less strong evidence: | Western blot,qRT-PCR | |
Target gene experiment description: | A distal Enhancer(DE) centred at ? 20 kb, when combined with a ? 2.5k proximal pro_x0002_moter, can also recapitulate human MyoD expression in vivo,demonstrating its critical role in determining the spatiotemporal expression of human MyoD. |
TF name : | Wnt3a(Wnt-3a,vt) | |
TF experiment: | Luciferase Reporter Assay | |
TF experiment description: | Serial deletion mutants of DE region were generated later to identify the minimal WRE,and we found that a region of 3 kb (fragment 10, −24 to −21 kb)in DE was required to retain the full Wnt3a response.Deletion of sequence at either the 5_x0002_ end (fragment 8) or the 3_x0002_ end(fragment 7) significantly reduced its Wnt3a response, comparedwith fragment. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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