Enhancer ID: | E_02_117 | |
Enhancer symbol: | ARC-specific Enhancer | |
Species: | Mouse | |
Position : | chr1:133303739-133325138 |
|
Biosample name: | Kisspeptin-Immunoreactive Cell | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 25486239 |
Enhancer experiment: | DNase-seq,Immunofluorescence,Luciferase Reporter Assay,PCR,3C | |
Enhancer experiment description: | Using open Chromatin mapping by DNase-seq in HTE cell,the core of DHS-44 was localized to 600 at hg 19,Chr7:117075400-117076000.This region was marked by enrichment of H3K27Ac in 16HBE14o-cell,consistent with DHS-44 encompassing an active Enhancer element. |
Target gene : | Kiss1(kisspeptin,metastatin) | |
Strong evidence: | 3C | |
Less strong evidence: | Transgenic mice | |
Target gene experiment description: | The present study aimed to determine ARC-specific enhancer(s) of the Kiss1 gene by an in vivo reporter assay.The 3C assay was performed to detect interactions between the Kiss1 promoter region and the 5 -upstream region, the latter of which was identified as an ARCspecific enhancer in the present study |
TF name : | Eral1(2610524P08Rik,9130407C09Rik,AU019798,Era,M-ERA,MERA-S,MERA-W) | |
TF experiment: | ChIP | |
TF experiment description: | The binding of ERa in the 5' upstream region (Figure 4) was determined by a ChIP assay with ERa antibody. |
Enhancer function : | The 5'-upstream region of Kiss1 locus functions as an enhancer for ARC Kiss1 gene expression in mice. |
Enhancer function experiment: | 3C |
Enhancer function experiment description: |
In addition,the current 3C and ER ChIP assays suggest that the Chromatin loop formation between the ARC-specific Enhancer and the promoter region of Kiss1 gene and unoccupied ER recruitment to the putative Enhancer region are involved in Kiss1 gene expression in the ARC |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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