Enhancer ID: | E_02_102 | |
Enhancer symbol: | -- | |
Species: | Mouse | |
Position : | chr17:35502632-35504632 |
|
Biosample name: | Embryonic Stem Cell | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 25223790 |
Enhancer experiment: | Luciferase Reporter Assay,ChIP,ChIP-qPCR | |
Enhancer experiment description: | We first investigated activation of the Oct4 enhancer by luciferase reporter assay 48 h after transfection of TALE-A and dCas9-A/gRNA in MEFs. These luciferase constructs contain the 2.4 kb region covering all three upstream regulatory elements of the Oct4 locus |
Target gene : | Oct4 | |
Strong evidence: | CRISPR/Cas9 | |
Less strong evidence: | qRT-PCR,Luciferase Reporter Assay | |
Target gene experiment description: | The Oct4 luciferase assay reporter constructs carried the genomic DNA 2.4 kb upstream of the Oct4 transcrip_x0002_tion start site (TSS). The region encompasses the 1.7 kb distal and proximal Enhancers and the 0.2 kb promoter. |
TF name : | Klf4(EZF,GKLF)Oct4Nanog(NANOG)Sox2(ANOP3,MCOPS3) | |
TF experiment: | ChIP-seq | |
TF experiment description: | To address this possibility, we reviewed the ChIP-seq information of several pluripotency transcription factors, including KLF4,OCT4,NANOG and SOX2 at the Nanog 5kb upstream Enhancer region(42) and found that the Site 2 (targeted by both TALE and dCas9) was surrounded by the predicted KLF4 and NANOG binding sites. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
-- |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
Home | Browse | Search | Download | Genome-Browser | Submit | Contact | Help
Copyright © HMU | 黑ICP备16009434号-1 | Li C Lab