Enhancer ID: | E_02_050 | |
Enhancer symbol: | -- | |
Species: | Mouse | |
Position : | chr14:67741735-67744661 |
|
Biosample name: | GT1-7 | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | <2KB | |
Pubmed ID: | 22761896 |
Enhancer experiment: | Luciferase Reporter Assay,ChIP,PCR | |
Enhancer experiment description: | the luciferase activities in GT1–7 cells were noticeably decreased without HDACIs treatments, suggesting that potential enhancer elements located in the regions (-3446,-2087 bp and -1134,-520 bp). |
Target gene : | Gnrh1(Gnrh,Gnrh2,LHRH,Lhrh1,Lnrh,hpg) | |
Strong evidence: | -- | |
Less strong evidence: | Western blot,Immunofluorescence,ChIP,EMSA | |
Target gene experiment description: | The Otx2 protein levels were also obviously reduced in GT1–7 cells showed by western blot analysis (Figure 5B) and immunofluorescence staining (Figure 5C).The results of ChIP assay showed that the binding ability of Otx2 to neuron-specific elements (2356,2249 bp) in Gnrh1 promoter obviously decreased after HDACIs treatments (Figure 6B).To further analyze the affinities of Otx2 to the two conserved binding sites in mouse Gnrh1 promoter respectively, EMSA was performed with probes containing the 2268,2239 bp and 2330,2301 bp sequences [20](Figure 6C, above). |
TF name : | Otx2(E130306E05Rik) | |
TF experiment: | ChIP,EMSA | |
TF experiment description: | Chromatin immunoprecipitation and electrophoretic mobility shift assays demonstrated that the DNA-binding activity of Otx2 to Gnrh1 gene was suppressed by TSA and VPA. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
-- |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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