About Enhancer
| Enhancer ID: | E_02_032 | |
| Enhancer symbol: | u-PAR Enhancer | |
| Species: | Mouse | |
| Position : | chr7:24461573-24462573   |
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| Biosample name: | Colon Cancer Cell | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | <2kb | |
| Pubmed ID: | 21937508 |
| Enhancer experiment: | RT-PCR,ChIP | |
| Enhancer experiment description: | In order to gain insight into the epigenetic regulation of u-PAR expression, ChIP assays were employed in an unbiased screen to identify histone modifications or exchange of histone variants at the u-PAR promoter and enhancer under conditions of gene activation.Quantitative real-time PCR (qPCR) was performed using chromatin generated from either unstimulated or PMA-stimulated GEO cells using primer sets corresponding to the promoter, enhancer and intron 3 (Figure 1A and C). |
About Target gene
| Target gene : | Plaur(Cd87,u-PAR,uPAR) | |
| Strong evidence: | -- | |
| Less strong evidence: | RT-PCR,ChIP | |
| Target gene experiment description: | In order to gain insight into the epigenetic regulation of u-PAR expression, ChIP assays were employed in an unbiased screen to identify histone modifications or exchange of histone variants at the u-PAR promoter and enhancer under conditions of gene activation.Quantitative real-time PCR (qPCR) was performed using chromatin generated from either unstimulated or PMA-stimulated GEO cells using primer sets corresponding to the promoter, enhancer and intron 3 (Figure 1A and C). |
About TF
| TF name : | Jun(AP-1,AP1,c-Jun,cJUN,p39) | |
| TF experiment: | ChIP | |
| TF experiment description: | AP-1 binding to the u-PAR gene precedes H2A.Z eviction. ChIP assay showing the kinetics of (A) AP-1 (using a mixture of pan antibodies directed against Fos and Jun family members) (B) H2A.Z (C) RNAP II (using an antibody against RNAP II phosphorylated at Serine 5) deposition at the u-PAR promoter and enhancer in response to 100?nm PMA for the indicated times. |
About Function
| Enhancer function : | The role of an AP-1-harboring intragenic Enhancer in the regulation of both constitutive and inducible u-PAR expression. |
| Enhancer function experiment: | ChIP |
| Enhancer function experiment description: |
In order to gain insight into the epigenetic regulation of u-PAR expression, ChIP assays were employed in an unbiased screen to identify histone modifications or exchange of histone variants at the u-PAR promoter and Enhancer under conditions of gene activation. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
