About Enhancer
| Enhancer ID: | E_02_026 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr5:147267701-147267751   |
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| Biosample name: | HIT,Hep G2,INS-1 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Diabetes Mellitus | |
| DO: | DOID:9351 | |
| Mesh: | D003920 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 21652712 |
| Enhancer experiment: | Transfection,Luciferase Reporter Assay,EMSA,ChIP,qPCR | |
| Enhancer experiment description: | Previous studies have identified several transcriptional factors for the Pdx-1 promoter that consists of two important regulatory regions as follows: the proximal and distal enhancer regions.To delineate the sites responsible for SREBP-lc suppression oí Pdx-1, we generated a series of 5'-deletion constructs and analyzed these reporters by transfection into HIT cells (Fig.2C). SREBP-lc-mediated inhibition was completely abolished In PDX-1 (-93)-Luc. |
About Target gene
| Target gene : | Pdx1(IDX-1,IPF-1,Ipf1,Mody4,STF-1,pdx-1) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay,EMSA | |
| Target gene experiment description: | SREBP-lc-mediated inhibition was completely abolished In PDX-1 (-93)-Luc. In the distal enhancer region, EMSA analysis showed that potent binding of PDX-1 to the probe containing a PDX-1 -binding site in area I (-2517 to - 2492 bp) was dose-dependently inhibited by SREBP-lc (Fig. 6） |
About TF
| TF name : | Pdx1(GSF,IDX-1,IPF1,IUF1,MODY4,PAGEN1,PDX-1,STF-1) | |
| TF experiment: | EMSA | |
| TF experiment description: | In the distal Enhancer region,EMSA analysis showed that potent binding of PDX-1 to the probe containing a PDX-1 -binding site in area I (-2517 to - 2492 ) was dose-dependently inhibited by SRE -lc. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
