Enhancer ID: | E_02_021 | |
Enhancer symbol: | -- | |
Species: | Mouse | |
Position : | chr14:67745496-67746000 |
|
Biosample name: | GT1-1 | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | <2KB | |
Pubmed ID: | 21527504 |
Enhancer experiment: | Luciferase Reporter Assay,Transfection,ChIP,qRT-PCR,EMSA | |
Enhancer experiment description: | To examine the enhancer activity of intron A, we constructed reporter vectors containing the full-length mouse intron A sequence (1003 bp) in either the forward or reversed direction followed by the simian vacuolating virus 40 (SV40) minimal promoter (SV40p) driving luciferase expression.These constructs were transfected into GnRH-expressing GT1-1 cells, and luciferase activity was then measured. Cells harboring the vectors with the full-length intron A exhibited luciferase activity significantly increased by 2- to 3-fold compared with those harboring only the SV40p (the “empty vector” in this study) (Fig. 1B). These findings strongly suggest that GnRH intron A contains a putative transcriptional enhancer that is likely involved in GnRH gene regulation. |
Target gene : | Gnrh1(Gnrh,Gnrh2,LHRH,Lhrh1,Lnrh,hpg) | |
Strong evidence: | -- | |
Less strong evidence: | Luciferase Reporter Assay | |
Target gene experiment description: | Schematic diagrams of the reporter constructs and their relative luciferase activities in comparison with those of SV40p-Ex1-Luc and SV40p-Ex1IA-315-Luc are shown in Fig. 4. Thus, these results strongly suggest that the region between 12 and 25 nt in the mouse GnRH intron A harbors a key cis-element for the enhancer function. |
TF name : | Sox4(AA682046,Sox-4)Sox11(1110038H03Rik,6230403H02Rik,AI836553,end1)Sox12 | |
TF experiment: | ChIP,EMSA | |
TF experiment description: | Chromatin immunoprecipitation (ChIP) assay re vealed that SOX11 binds to the Ex1-proximal Enhancer region of intron A, and overexpression of SOX11 in creases this binding in GT1-1 cells.In accor_x005f_x0002_dance with the result in the reporter assay, EMSA revealed that in vitro translated SOX4 and SOX11 proteins can recognize both SOX-binding sites in mouse intronA; the binding was completely abrogated when both sites were mutated but partially diminished in the probes with a deletion of one of them. |
Enhancer function : | Class-C SOX Transcription Factors Control GnRH Gene Expression via the Intronic Transcriptional Enhancer. |
Enhancer function experiment: | Luciferase Reporter Assay,ChIP,EMSA |
Enhancer function experiment description: |
Chromatin immunoprecipitation shows that endogenous SOX-C factors recognize and bind to the intronic Enhancer in GT1-1 cells and the hypothalamus. Taken together,these findings demonstrate that SOX-C transcription factors function as important transcriptional regulators of cell type-specific GnRH gene expression by acting on the intronic transcriptional Enhancer. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
Home | Browse | Search | Download | Genome-Browser | Submit | Contact | Help
Copyright © HMU | 黑ICP备16009434号-1 | Li C Lab