About Enhancer
| Enhancer ID: | E_02_020 | |
| Enhancer symbol: | mRL-D5 Enhancer | |
| Species: | Mouse | |
| Position : | chr14:78351421-78354313   |
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| Biosample name: | T Cell | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 21487009 |
| Enhancer experiment: | ChIP-chip,3C,ChIP,Luciferase Reporter Assay,PCR | |
| Enhancer experiment description: | We used ChIP-chip analysis in mouse primary T cells and a T cell hybridoma to define the regulatory enhancers responsible for this up-regulation and to characterize their properties.Our previous ChIP-chip analyses of the mouse Rankl locus suggested that the long distance Rankl enhancers were routinely marked in osteoblasts by both basal and 1,25(OH)2D3-, PTH-, or cytokine-inducible levels of H4ac as well as increased RNA pol II density. |
About Target gene
| Target gene : | Tnfsf11(CD254,ODF,OPGL,OPTB2,RANKL,TNLG6B,TRANCE,hRANKL2,sOdf) | |
| Strong evidence: | 3C | |
| Less strong evidence: | -- | |
| Target gene experiment description: | To determine whether looping might provide a mechanism for Rankl activation via the mRL-D5 and the TCCR regions, we treated 2b4.11 cells with either vehicle or PI and used 3C analysis to assess potential interactions between the promoter and enhancers as described under “Experimental Procedures.” |
About TF
| TF name : | Fos(AP-1,C-FOS, p55)Rela(p65,p65 NF-kappa B,p65 NFkB) | |
| TF experiment: | 3C,ChIP | |
| TF experiment description: | They also indicate that despite our inability to detect its presence in the ChIP-ChIP analysis,Nfat or related transcription factor activation may be involved in transcriptional regulation through the putative mRL-T1A Enhancer. |
About Function
| Enhancer function : | mRL-D5 and segments of the TCCR were located in proximity to the Rankl gene promoter and thus potentially able to influence directly Rankl gene promoter activity. |
| Enhancer function experiment: | 3C,ChIP,Luciferase Reporter Assay,PCR |
| Enhancer function experiment description: |
These data also suggest that osteoblasts and T cell both may utilize a common Enhancer such as mRL-D5 and cell type-specific Enhancers such as the TCCR to regulate Rankl gene expression. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
