About Enhancer
| Enhancer ID: | E_02_017 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chrx:7954360-7956360   |
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| Biosample name: | Embryonic Stem Cell | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 21464046 |
| Enhancer experiment: | Luciferase Reporter Assay,RT-PCR | |
| Enhancer experiment description: | The 3.9 kb Gata1 hematopoietic enhancer and minigene was previously described.We have previously generated a transgenic mouse model using an Nkx2-5 cardiac specific enhancer to direct EYFP reporter expression to the developing heart.Transcriptional assay in K562 cells reveal a dose-dependent repression of luciferase activity by Nkx2-5 using the 3.9 kb erythroid enhancer. |
About Target gene
| Target gene : | Gata1(ERYF1,GATA-1,GF-1,GF1,NF-E1,NFE1,XLANP,XLTDA,XLTT) | |
| Strong evidence: | -- | |
| Less strong evidence: | Transfection | |
| Target gene experiment description: | Utilizing transgenic technologies to isolate Nkx2-5 expressing cells, we observed an induction of the erythroid molecular program, including Gata1, in the Nkx2-5 null embryos. We demonstrated that Nkx2-5 binds to the Gata1 gene enhancer and represses the transcriptional activity of the Gata1 gene. |
About TF
| TF name : | Nkx2-5(Csx,Nkx-2.5,Nkx2.5,tinman) | |
| TF experiment: | Luciferase Reporter Assay | |
| TF experiment description: | Using transcriptional assays,we showed that one of the targets of Nkx2-5 is the Gata1 gene,an essential transcription factor in erythroid differentiation. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
