About Enhancer
| Enhancer ID: | E_02_015 | |
| Enhancer symbol: | -- | |
| Species: | Mouse | |
| Position : | chr14:67742375-67743103   |
|
| Biosample name: | Epithelial Cell,Granulosa Cells,GT1-7,GN11,Embryonic Stem Cell | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 21368045 |
| Enhancer experiment: | RT-PCR,Luciferase Reporter Assay | |
| Enhancer experiment description: | Furthermore, studies in mice bearing either −3446/+23 or −2078/+23 bp of the mGnRH promoter fused to the LUC reporter defined a critical enhancer region for the in vivo expression of hypothalamic mGnRH (Kim et al., 2002).The quantitative RT-PCR primer sequences are available on request. |
About Target gene
| Target gene : | Gnrh1(Gnrh,Gnrh2,LHRH,Lhrh1,Lnrh,hpg) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay | |
| Target gene experiment description: | In our previous study, using the luciferase (LUC) reporter gene, we found that the proximal −1005 bp of the mouse GnRH (mGnRH) promoter contains the critical elements for appropriate neuronal and ovarian expression of mGnRH (Kim et al., 2002). Furthermore, studies in mice bearing either −3446/+23 or −2078/+23 bp of the mGnRH promoter fused to the LUC reporter defined a critical enhancer region for the in vivo expression of hypothalamic mGnRH (Kim et al., 2002). |
About TF
| TF name : | Oct1Gata4(ASD2,TACHD,TOF,VSD1) | |
| TF experiment: | Luciferase Reporter Assay | |
| TF experiment description: | Oct-1 and GATA-4 were identified as important transcription factors that bind to the neuron-specific Enhancer and regulate GnRH expression in vitro. |
About Function
| Enhancer function : | This work supports the hypothesisthatthe region ofthe GnRH promoter contained between -2806 and -2078 bp acts as a cell-specific Enhancer in the GnRH neuron and as a repressor in the ovary. |
| Enhancer function experiment: | Luciferase Reporter Assay |
| Enhancer function experiment description: |
In this work, to further characterize the mGnRH promoter,we have constructed an additional transgenic mouse bearing a -2806/+23 bp fragment fused to the LUC reporter gene. This model demonstrates that critical hypothalamic Enhancer se_x005f_x0002_quences and ovarian repressor elements for the in vivo expression of mGnRH are located between -2806 and -2078 bp on the mGnRH promoter. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
