Enhancer ID: | E_02_012 | |
Enhancer symbol: | Slc30a8 Enhancer | |
Species: | Mouse | |
Position : | chr15:52315678-52316298 |
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Biosample name: | Islet B-Cell | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | Intron | |
Pubmed ID: | 20942803 |
Enhancer experiment: | Luciferase Reporter Assay,PCR | |
Enhancer experiment description: | To address this hypothesis these regions, designated intronic enhancers A and B, respectively, were isolated using PCR and ligated 5′ of a heterologous thymidine kinase (TK)-luciferase fusion gene containing TK genomic sequence between −105 and +51, relative to the transcription start site.Luciferase expression directed by these fusion genes was then analyzed by transient transfection of βTC-3 cells, an islet beta cell-derived line [31], αTC-6 cells, an islet alpha cell-derived line [32, 33] and HeLa cells, a cervix-derived cell line. |
Target gene : | Slc30a8(ZNT8,ZnT-8) | |
Strong evidence: | -- | |
Less strong evidence: | PCR,Luciferase Reporter Assay | |
Target gene experiment description: | Luciferase expression directed by these fusion genes was then analyzed by transient transfection of βTC-3 cells, an islet beta cell-derived line [31], αTC-6 cells, an islet alpha cell-derived line [32, 33] and HeLa cells, a cervix-derived cell line. Figure 2 shows that in βTC-3 cells, but not αTC-6 or HeLa cells, intronic enhancer A elevated reporter gene expression beyond that driven by the TK-luciferase fusion gene alone, indicating that this region is an islet beta cell-specific enhancer. In contrast, intronic enhancer B had no effect on fusion gene expression in any of the cell lines. |
TF name : | Pbx1(2310056B04Rik,D230003C07Rik,Pbx-1) | |
TF experiment: | ChIP,EMSA | |
TF experiment description: | Gel retardation and chromatin immunoprecipitation (ChIP) assays revealed that the islet-enriched transcription factor Pdx-1 binds Enhancer A in vitro and in situ, respectively. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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