Enhancer ID: | E_02_007 | |
Enhancer symbol: | CNS-2 Enhancer | |
Species: | Mouse | |
Position : | chr3:37225520-37228865 |
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Biosample name: | CD4+ T Cell | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 20042469 |
Enhancer experiment: | Luciferase Reporter Assay,ChIP-qPCR,ChIP,PCR | |
Enhancer experiment description: | To determine the function of IL-21P and CNS-2 in the transcriptional control of IL-21 expression by c-Maf, we performed a reporter assay. IL-21P-Luci containing a fragment of IL-21P (positions –261 to 17), IL-21P-Luci-CNS-2 containing IL-21P and CNS-2 (position +2931 to +3443), or control pGL3 was transfected into EL4 cells, together with pcDNA3 c-Maf or control pcDNA3, and the luciferase activity of the reporter construct was evaluated with or without PMA+I stimulation. As shown in Figure3B, IL-21P-Luci responded to PMA+I stimulation, and c-Maf enhanced the activity of IL-21P-Luci (n=3; P<0.05) further. IL-21P-Luci-CNS-2 responded much greater to c-Maf in the presence of PMA+I stimulation (Fig. 3B). These results suggest that CNS-2 functions as an enhancer of the IL-21 gene and that c-Maf activates IL-21P as well as CNS-2.After cells were stimulated with or without PMA+I for 1 h, ChIP-qPCR assay for IL-21P, CNS-2, and CNS-4 was performed with anti-c-Maf anti-body or control rabbit IgG. Results are expressed as the percent input for each ChIP fraction. |
Target gene : | Il21(IL-21) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP | |
Target gene experiment description: | To determine whether c-Maf actually binds to IL-21P and the CNS-2 enhancer in CD4 + T cells, we performed ChIP analysis.These results suggest that CNS-2 functions as an Enhancer of the IL-21 gene and that c-Maf acti_x005F_x0002_vates IL-21P as well as CNS-2. |
TF name : | Maf(2810401A20Rik,A230108G15Rik,AW047063,c-maf) | |
TF experiment: | ChIP | |
TF experiment description: | To determine whether c-Maf actually binds to IL-21P and the CNS-2 Enhancer in CD4 T cells, we performed ChIP analysis. |
Enhancer function : | c-Maf induces IL-21 production directly in CD4_x005f_x0002_ T cells by activating IL-21P and the CNS-2 Enhancer and that TGF-β suppresses c-Maf-induced IL-21 production in CD4_x0002_ T cells. |
Enhancer function experiment: | Luciferase Reporter Assay,PCR |
Enhancer function experiment description: |
Taken together with the data shown in Figure 3, these results suggest that c-Maf binds di_x005f_x005f_x005f_x005F_x005f_x005F_x005f_x005F_x0002_rectly to IL-21P and the CNS-2 Enhancer in CD4_x005F_x0001_ T cells after IL-6 stimulation and that TGF-_x0001_ enhances IL-6-induced c-Maf binding to IL-21P and the Enhancer. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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