About Enhancer
| Enhancer ID: | E_01_420 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr14:24435744-24437744   |
|
| Biosample name: | Hep G2,HL-7702 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 26864944 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP | |
| Enhancer experiment description: | we searched for and identified a putative enhancer region located 13.8 kb downstream from the TSS of DHRS4-AS117,18. We performed chromatin immunoprecipitation (ChIP) to confirm the enrichment of histone modifications in hepatocellular carcinoma cells (HepG2) and normal hepatocytes (HL7702) (Fig. 1D). We then examined the potential enhancement of luciferase reporter activity by using luciferase reporter assays in HepG2 and HL7702 cells (Fig. 1E). |
About Target gene
| Target gene : | DHRS4-AS1(AS1DHRS4,C14orf167,C14orf67,DHRS4AS1,PRO1488) | |
| Strong evidence: | 3C | |
| Less strong evidence: | Luciferase Reporter Assay,ChIP,qPCR | |
| Target gene experiment description: | In a search of the DHRS4-AS1 region for nearby enhancers, we identified an enhancer located 13.8 kb downstream of the DHRS4-AS1 transcriptional start site. We further showed, by using a chromosome conformation capture (3C) assay, that this enhancer is capable of physically interacting with the DHRS4-AS1 promoter through chromosomal looping. |
About TF
| TF name : | EP300(KAT3B,MKHK2,RSTS2,p300) | |
| TF experiment: | ChIP | |
| TF experiment description: | RNA pol II and the transcriptional coactivator p300/CBP occupy enhancer regions and target gene promoters to maintain chromatin loops29. We next characterized the effect of AS1eRNA knockdown on the binding levels of RNA pol II and p300 at the AS1 enhancer and the DHRS4-AS1 promoter (Fig. 4A,B). |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
