Enhancer ID: | E_01_419 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr8:29203575-29214287 |
|
Biosample name: | MCF-7,MDA-MB-231 | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | Breast Cancer | |
DO: | DOID:1612 | |
Mesh: | D001943 | |
Distance from TSS: | >2KB | |
Pubmed ID: | 26859151 |
Enhancer experiment: | ChIP-seq | |
Enhancer experiment description: | DUSP4 co-localised with the enhancer mark H3K27ac [40] in MCF-7/PMA (PCC = 0.40) but not in non-stimulated MCF-7 cells (PCC = -0.30). Moreover, DUSP4 co-localised with the enhancer mark H3K4me1 in MCF-7 cells (PCC = 0.35), which increased in MCF-7/PMA cells (PCC = 0.44) (Fig 2D). In comparison, there was absent or minimal co-localisation of DUSP4 with H3K4me3, H3K4me1, and H3K9me3 in MCF-7 and MCF-7/PMA cells (Fig B in S2 Fig). Conversely, DUSP6 was expressed at very low levels or did not co-localise with any of the tested histone modifications in MCF-7 or MCF-7/PMA cells (Fig A in S3 Fig). |
Target gene : | DUSP4(HVH2,MKP-2,MKP2,TYP) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP,RT-qPCR | |
Target gene experiment description: | Similarly, DUSP4 was enriched at the promoters of these genes in MCF-7 cells and dis_x0002_played reduced enrichment in MCF-7/PMA cells at PLAUR and IL6 but not FN1 gene promot_x0002_ers (Fig 3B).Furthermore, DUSP4 half_x0002_ChIP on MCF-7 and MCF-7/PMAAD nuclear extracts showed that DUSP4 associates with both key phosphorylation residues (Ser2 and Ser5) of a key indicator of active chromatin, RNA Polymerase II (Fig 3C). |
TF name : | EP300(KAT3B,MKHK2,RSTS2,p300) | |
TF experiment: | Immunofluorescence,Half-ChIP,RT-qPCR | |
TF experiment description: | Immunofluoresence anal_x0002_ysis showed that the nuclear fluorescence intensity of p300 increased in the mesenchymal state,in MCF-7/PMASUS and MDA-MB-231 cells compared to MCF-7 cells (Fig 4A). Moreover,DUSP4 and p300 significantly co-localise in MCF-7/PMASUS (PCC = 0.42) and MDA-MB-231 cells (PCC = 0.50) and minimally co-localised in MCF-7 cells (PCC = 0.15) (Fig 4A). Consis_x0002_tent with these findings, DUSP4 half-ChIP on MCF-7 and MCF-7/PMAAD nuclear extracts showed that DUSP4 increased its association with p300 in the mesenchymal state (S4 Fig).Next, we investi_x0002_gated the impact of DUSP4 knockdown on p300 phosphorylation by immunofluorescence analysis of either MOCK or validated DUSP4 siRNA-treated MCF-7 cells prior and subsequent to stimulation with PMA. DUSP4 knockdown was first confirmed by real-time PCR and immunoblotting (Figs A, B, C in S5 Fig). |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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