About Enhancer

Enhancer ID: E_01_419
Enhancer symbol: --
Species: Human
Position : chr8:29203575-29214287
Biosample name: MCF-7,MDA-MB-231
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: Breast Cancer
DO: DOID:1612
Mesh: D001943
Distance from TSS: >2KB
Pubmed ID:  26859151
Enhancer experiment: ChIP-seq
Enhancer experiment description: DUSP4 co-localised with the enhancer mark H3K27ac [40] in MCF-7/PMA (PCC = 0.40) but not in non-stimulated MCF-7 cells (PCC = -0.30). Moreover, DUSP4 co-localised with the enhancer mark H3K4me1 in MCF-7 cells (PCC = 0.35), which increased in MCF-7/PMA cells (PCC = 0.44) (Fig 2D). In comparison, there was absent or minimal co-localisation of DUSP4 with H3K4me3, H3K4me1, and H3K9me3 in MCF-7 and MCF-7/PMA cells (Fig B in S2 Fig). Conversely, DUSP6 was expressed at very low levels or did not co-localise with any of the tested histone modifications in MCF-7 or MCF-7/PMA cells (Fig A in S3 Fig).

About Target gene

Target gene : DUSP4(HVH2,MKP-2,MKP2,TYP)
Strong evidence: --
Less strong evidence: ChIP,RT-qPCR
Target gene experiment description: Similarly, DUSP4 was enriched at the promoters of these genes in MCF-7 cells and dis_x0002_played reduced enrichment in MCF-7/PMA cells at PLAUR and IL6 but not FN1 gene promot_x0002_ers (Fig 3B).Furthermore, DUSP4 half_x0002_ChIP on MCF-7 and MCF-7/PMAAD nuclear extracts showed that DUSP4 associates with both key phosphorylation residues (Ser2 and Ser5) of a key indicator of active chromatin, RNA Polymerase II (Fig 3C).

About TF

TF name : EP300(KAT3B,MKHK2,RSTS2,p300)
TF experiment: Immunofluorescence,Half-ChIP,RT-qPCR
TF experiment description: Immunofluoresence anal_x0002_ysis showed that the nuclear fluorescence intensity of p300 increased in the mesenchymal state,in MCF-7/PMASUS and MDA-MB-231 cells compared to MCF-7 cells (Fig 4A). Moreover,DUSP4 and p300 significantly co-localise in MCF-7/PMASUS (PCC = 0.42) and MDA-MB-231 cells (PCC = 0.50) and minimally co-localised in MCF-7 cells (PCC = 0.15) (Fig 4A). Consis_x0002_tent with these findings, DUSP4 half-ChIP on MCF-7 and MCF-7/PMAAD nuclear extracts showed that DUSP4 increased its association with p300 in the mesenchymal state (S4 Fig).Next, we investi_x0002_gated the impact of DUSP4 knockdown on p300 phosphorylation by immunofluorescence analysis of either MOCK or validated DUSP4 siRNA-treated MCF-7 cells prior and subsequent to stimulation with PMA. DUSP4 knockdown was first confirmed by real-time PCR and immunoblotting (Figs A, B, C in S5 Fig).

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:
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About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs