About Enhancer
| Enhancer ID: |
|
E_01_400 |
| Enhancer symbol: |
|
HS2 enhancer |
| Species: |
|
Human |
| Position : |
|
chr11:5271421-5273421
   |
| Biosample name: |
|
K-562 |
| Experiment class : |
|
Low throughput |
| Enhancer type: |
|
enhancer |
| Disease: |
|
-- |
| DO: |
|
-- |
| Mesh: |
|
-- |
| Distance from TSS: |
|
>2kb |
| Pubmed ID: |
|
15371553 |
| Enhancer experiment: |
|
RNase Protection Assay |
| Enhancer experiment description: |
|
The minichromosomes were stably maintained in human erythroid K562 cells in which the endogenous e-globin gene is actively transcribed. RNase protection assays were performed to test the influence of the insulators on enhancer activation of the e-globin gene. |
About Target gene
| Target gene : |
|
HBE1(HBE) |
| Strong evidence: |
|
-- |
| Less strong evidence: |
|
Luciferase Reporter Assay |
| Target gene experiment description: |
|
The minichromosomes were stably maintained in human erythroid K562 cells in which the
endogenous e-globin gene is actively transcribed. RNase pro_x0002_tection assays were performed to test the influence of the insulators on enhancer activation of the ε-globin gene.Representative assays of RNA from different cHS4 insula_x0002_tor-containing clones are illustrated in Figure 1B, and the results for least 12 individual clones of each type are summar_x0002_ized in Figure 1C. Abundant transcription of the e-globin RNA occurred when the gene was linked to HS2. |
About TF
| TF name : |
|
NFE2(NF-E2,p45) |
| TF experiment: |
|
ChIP |
| TF experiment description: |
|
Therefore, we used antibodies to the HS2 enhancer factor NF-E2 and the insulator factor CTCF in ChIP assays of the cHS4in and cHS4out insulated loci. |
About Function
| Enhancer function : |
-- |
| Enhancer function experiment: |
-- |
Enhancer function
experiment description: |
-- |
About SNP
| SNP ID: |
|
-- |
| SNP position: |
|
-- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
Expression of target genes for the enhancer
Enhancer associated SNPs
