About Enhancer
| Enhancer ID: | E_01_398 | |
| Enhancer symbol: | ||
| Species: | Human | |
| Position : | chr11:5211019-5213983   |
|
| Biosample name: | K-562 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2kb | |
| Pubmed ID: | 17637499 |
| Enhancer experiment: | Luciferase Reporter Assay | |
| Enhancer experiment description: | Enhancer activity of reporter constructs was determined with the dual luciferase system in transient transfection assays. Notch signaling transactivated a reporter construct harboring a con_x0002_served RBP-J (CBF1) binding site in the hypersensitive site 2 (HS2) of human b-globin. Transactivation by activated Notch was completely abolished when this RBP-J site was mutated to prevent RBP-J binding. |
About Target gene
| Target gene : | HBB(CD113t-C,ECYT6,beta-globin) | |
| Strong evidence: | -- | |
| Less strong evidence: | RT-PCR,Northern blot | |
| Target gene experiment description: | Expression was analyzed by Northern blotting and real-time polymerase chain reac_x0002_tion.Notch signaling transactivated a reporter construct harboring a con_x0002_served RBP-J (CBF1) binding site in the hypersensitive site 2 (HS2) of human b-globin. |
About TF
| TF name : | RBPJ (AOS3,CBF1,IGKJRB,IGKJRB1,KBF2,RBP-JK,RBPSUH,SUH,csl,RBPJ) | |
| TF experiment: | Luciferase Reporter Assay | |
| TF experiment description: | Luciferase reporter constructs containing either the functional RBP-J binding site of the HS2 [38] or a mutated sequence (Fig. 5A) that does not bind RBP-J [53] or showed reduced RBP-J binding [38] were transiently transfected into rNERTneo and rneo FDCP-mix cells and luciferase activity was determined in the presence or absence of OHT. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
