About Enhancer
| Enhancer ID: | E_01_396 | |
| Enhancer symbol: | ||
| Species: | Human | |
| Position : | chr11:5250751-5250777   |
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| Biosample name: | K-562,HEL | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Chronic myelogenous leukemia | |
| DO: | DOID:8552 | |
| Mesh: | D015464 | |
| Distance from TSS: | >2kb | |
| Pubmed ID: | 10595394 |
| Enhancer experiment: | Luciferase Reporter Assay,EMSA | |
| Enhancer experiment description: | DNA footprinting analyses and electrophoretic mobility shift assays (EMSAs) indicate that two sites for which g PE has a high affinity, 3' γPE1 and 3' γPE2, span from +1992 to + 2023 and +2263 to +2288, respectively, and contain the consensus sequence ATTANNNGGAANNCT(N)NNNNTAATGG. Three low-affinity sites,3' γPE1 (+2245 to +2225),3' γPE3 (+2432 to +2447), and 3' γPE4 (+2479 to +2505),contain the consensus sequences 59 -AAAAN(T/A)A(A/T)TT-3'. |
About Target gene
| Target gene : | HBG1(HBG-T2,HBGA,HBGR,HSGGL1,PRO2979) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay | |
| Target gene experiment description: | A large nuclear protein complex, termed γPE (for γ-globin promoter and enhancer binding factor), binds to five sites located 5' and 3' of the human γ-globin gene.Transient cotransfections of a HOXB2 cDNA and a c -globin–luciferase reporter gene con_x0002_struct into cells expressing SATB1 suggested that SATB1 has a positive and HOXB2 a negative regulatory effect on transcription. |
About TF
| TF name : | SATB1(SATB1)HOXB2(HOX2,HOX2H,Hox-2.8,K8) | |
| TF experiment: | EMSA | |
| TF experiment description: | The present work showed that antisera directed against either SATB1 or HOXB2 reacted specifically with the entire γPE complex in electrophoretic mobility shift assays (EMSAs), suggesting that the two pro_x0002_teins can bind to the γPE binding site simultaneously. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
