About Enhancer
| Enhancer ID: | E_01_391 | |
| Enhancer symbol: | ||
| Species: | Human | |
| Position : | chr3:108296550-108297750   |
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| Biosample name: | K-562 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2kb | |
| Pubmed ID: | 9878258 |
| Enhancer experiment: | CAT Assay,PCR,RT-PCR | |
| Enhancer experiment description: | Functional tests with the CAT reporter gene assays demonstrate that the human 59 HS5 LTR activates the cis-linked CAT gene and possesses enhancer and pro_x0002_moter activities in erythroid cells.We found an LTR retrotranspo_x0002_son belonging to the ERV-9 family of human endoge_x0002_nous retroviruses in the apparent 5* boundary area of the LCR. This ERV-9 LTR contains an unusual U3 en_x0002_hancer region composed of 14 tandem repeats with recurrent GATA, CACCC, and CCAAT motifs. |
About Target gene
| Target gene : | HBB(CD113t-C,ECYT6,beta-globin) | |
| Strong evidence: | -- | |
| Less strong evidence: | CAT Assay,RT-PCR | |
| Target gene experiment description: | We next determined whether the endogenous 59 HS5 LTR also exhibits enhancer and promoter activities and can activate the transcription of the downstream R region and the flanking genomic DNA in the b-globin LCR.The above RT-PCR results indicate that the endog_x0002_enous 59 HS5 LTR possesses apparent enhancer and promoter activities and is capable of promoting the transcription of the R and U5 regions in the LTR and of further downstream genomic DNA in the LCR. |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | In both recombinant constructs and the endog_x0002_enous human genome, the LTR enhancer and promoter activate the transcription of cis-linked DNA preferentially in erythroid cells. |
| Enhancer function experiment: | CAT Assay |
| Enhancer function experiment description: |
Functional tests with the CAT reporter gene assays demonstrate that the human 59 HS5 LTR activates the cis-linked CAT gene and possesses enhancer and pro_x0002_moter activities in erythroid cells. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
