About Enhancer
| Enhancer ID: | E_01_387 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr11:5266021-5266708   |
|
| Biosample name: | K-562 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 10523648 |
| Enhancer experiment: | Transfection,RNase Protection Assay,DNase I Hypersensitivity Assay | |
| Enhancer experiment description: | We investigated the requirements for enhancer-promoter communication by using the human b-globin locus control region (LCR) DNase I-hypersensitive site 2 (HS2) enhancer and the Ɛepsilon-globin gene in chromatinized minichromosomes in erythroid cells.Activation of globin genes during development is accompanied by local_x0002_ized alterations of chromatin structure, and CACCC binding factors and GATA-1, which interact with both globin promoters and the LCR, are believed to be critical for globin gene transcription activation. |
About Target gene
| Target gene : | HBE1(HBE) | |
| Strong evidence: | -- | |
| Less strong evidence: | DNase I Hypersensitivity Assay | |
| Target gene experiment description: | We found that an HS2 element mutated in its GATA motif failed to remodel the «-globin promoter or activate tran_x0002_scription yet HS2 nuclease accessibility did not change. Accessibility and transcription were reduced at promoters with mutated GATA-1 or CACCC sites. Strikingly, these mutations also resulted in reduced accessibility at HS2. |
About TF
| TF name : | GATA1(ERYF1,GATA-1,GF-1,GF1,NF-E1,NFE1,XLANP,XLTDA,XLTT)NFE2(NF-E2,p45) | |
| TF experiment: | DNase I Hypersensitivity Assay | |
| TF experiment description: | Because of the reduced DNase I sensitivity of HS2 in the absence of a globin gene, cleavage at sites close to the HS2 GATA-1 and NF-E2 binding motifs could be visualized at the lowest enzyme concentration.However, DNase I sensi_x0002_tivity at HS2 was reduced when either the promoter 2165 GATA site or the CACCC site was mutated.Aquantitative assessment of these changes by MscI digestion of nuclei revealed that accessibility at HS2 for these promoter mutants was reduced to about half and differed significantly from the wild-type level (P,0.05). Thus, the struc_x0002_tural effect on HS2 of each of these mutations is equivalent to the effect of removing the globin gene entirely. |
About Function
| Enhancer function : | Further, at least in this instance, transcription activation and promoter remodeling by a distant enhancer are not separable. |
| Enhancer function experiment: | DNase I Hypersensitivity Assay |
| Enhancer function experiment description: |
To determine whether the HS2 mutations affected transcription, ε-globin RNA levels were measured by RNase protection as illustrated in Fig. 4A. The minichromosomal ε-globin gene is not transcribed in the absence of an enhancer, while inclusion of HS2 results in transcription of the linked gene.The results suggest that in chromatin,multiple interactions contribute to enhancer-promoter communication. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
