Enhancer ID: | E_01_386 | |
Enhancer symbol: | ||
Species: | Human | |
Position : | chr11:5268575-5268621 |
|
Biosample name: | K-562 | |
Experiment class : | low throughput |
Enhancer type: | enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | -- | |
Pubmed ID: | 2339058 |
Enhancer experiment: | DNase I Hypersensitivity Assay | |
Enhancer experiment description: | A 46 bp fragment was made that contained this sequence (46bpE) and a 1.5 kb fragment was made (HS II-E) that was identical to the wildtype HS II fragment except that a portion of the minimal enhancer was replaced by a plasmid polylinker ablating both API sites (fig.7). This data clearly shows that the 46 bp minimal enhancer is both necessary and sufficient for gamma-globin gene induction with hemin induced erythroid maturation in K562 cells. |
Target gene : | HBG1(HBG-T2,HBGA,HBGR,HSGGL1,PRO2979) | |
Strong evidence: | -- | |
Less strong evidence: | DNase I Hypersensitivity Assay,Southern blot,PCR | |
Target gene experiment description: | To determine the effect of fragments containing LAR sequences on globin gene expression, mRNA from a marked gamma_x0002_globin gene linked to LAR fragments was assayed in stably transfected K562 erythroleukemia cells. DNasel hypersensitive site 11 (HS 11), located 10.9 kb upstream of the epsilon-globin gene, was required for high level gamma-globin gene expression. |
TF name : | -- | |
TF experiment: | -- | |
TF experiment description: | -- |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
-- |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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