About Enhancer
| Enhancer ID: | E_01_377 | |
| Enhancer symbol: | ES-specific enhancers (ESSEs) | |
| Species: | Human | |
| Position : | chr6:43891400-43898360   |
|
| Biosample name: | K-562 | |
| Experiment class : | Low+High throughput |
| Enhancer type: | enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2kb | |
| Pubmed ID: | 26886256 |
| Enhancer experiment: | ChIP-seq | |
| Enhancer experiment description: | We termed these promoter-distal elements, which carry the H3K4me1 mark in ES but not in hematopoietic cells,the ES-specific enhancers (ESSEs) of the hematopoietic cell lineage. |
About Target gene
| Target gene : | VEGFA (MVCD1,VEGF,VPF) | |
| Strong evidence: | 4C-seq,CRISPR/Cas9 | |
| Less strong evidence: | Luciferase Reporter Assay | |
| Target gene experiment description: | To determine whether this ESSE is able to enhance transcription, a 1,500 bp fragment surrounding the ESSE methylation site was cloned upstream to a minimal SV40 promoter in a luciferase reporter plasmid and transfected to K562 cells.Indeed, the ESSE segment significantly enhanced expression when placed upstream or downstream to the minimal promoter. Thus,the DNA element surrounding the ESSE site is a transcriptional enhancer in leukemia cells.The Circularized Chromosome Conformation Capture (4C-seqencing) assay allowed mapping the interactions between the VEGFA promoter and DNA sequences across the K562 cell genome.To determine whether VEGFA expression depends upon the ESSE, we mutated the ESSE site in K562 cells by utilization of the CRISPR/Cas9 genome-editing system. |
About TF
| TF name : | STAT1/2(CANDF7,IMD31A,IMD31B,IMD31C,ISGF-3,STAT91) | |
| TF experiment: | ChIP-seq | |
| TF experiment description: | Focused (<600 bp) cluster of transcription factor binding sites (Chip-seq data) within the predicted enhancer. The factors bound in K562 cells are listed. This is the only cluster within 20 kb around the ESSE. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
