About Enhancer
| Enhancer ID: | E_01_353 | |
| Enhancer symbol: | POU5F1 naive Enhancer | |
| Species: | Human | |
| Position : | chr6:31137056-31138028   |
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| Biosample name: | Human Primordial Germ Cell | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 30590035 |
| Enhancer experiment: | ATAC-seq,ChIP-seq | |
| Enhancer experiment description: | These data show that the PE, DE, and NE are all open in hPGCLCs at day 4 of aggregate differentiation, with the NE being more open in hPGCs than the DE and PE. This observation raises the possibility that restriction of OCT4 expression between days 3 and |
About Target gene
| Target gene : | POU5F1(OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP-qPCR | |
| Target gene experiment description: | Screenshot of ATAC-seq and TFAP2C ChIP-seq signals showing three Enhancers at the POU5F1 locus (encoding OCT4). Shaded boxes highlight the naive Enhancer(NE),proximalEnhancer(PE),anddistalEnhancer(DE)atthePOU5F1locus.DEdeletion andNEdeletion indicategenomicregionsthatweredeleted by CRISPR/Cas9-mediated genome editing. Primers for ChIP-qPCR (P1-2,P3-4,P5-6) of NE and control regions are shown. |
About TF
| TF name : | TFAP2C(AP2-GAMMA,ERF1,TFAP2G,hAP-2g) | |
| TF experiment: | CRISPR/Cas9 | |
| TF experiment description: | Using CRISPR/Cas9, we show that deleting the TFAP2C-bound naive Enhancer at the OCT4 locus (also called POU5F1) results in impaired OCT4 expression and a negative effect on hPGCLC identity. |
About Function
| Enhancer function : | The PE, DE, and NE are all open in hPGCLCs at day 4 of aggregate differentiation. |
| Enhancer function experiment: | ATAC-seq |
| Enhancer function experiment description: |
First, to determine whether the NE is also open in human germline cells, we compared ATAC-seq peaks for the NE in hPGCLCs and hPGCs to this region in naive and primed hESCs (Figure 5C). These data show that the PE, DE, and NE are all open in hPGCLCs at day 4 of aggregate differentiation, with the NE being more open in hPGCs than the DE and PE. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
