About Enhancer
| Enhancer ID: | E_01_333 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr16:86422446-86426834   |
|
| Biosample name: | HEK-293 | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 30119690 |
| Enhancer experiment: | ChIP-seq,Luciferase Reporter Assay,CRISPR/Cas9 | |
| Enhancer experiment description: | Notably, two of these five sgRNAs,sgRNAs-AP169 and sgRNA-AP171, are independent sgRNAs that target the same enhancer region,hence increasing the confidence that these are true-positive hits. ENCODE ChIP-seq data confirmed a strong binding of both FOS and JUN to this region. |
About Target gene
| Target gene : | FOXF1(ACDMPV,FKHL5,FREAC1) | |
| Strong evidence: | CRISPR/Cas9 | |
| Less strong evidence: | qRT-PCR | |
| Target gene experiment description: | Furthermore,we identified FOXF1 as the tar_x0002_get gene of this Enhancer and demonstrated that it regu_x0002_lates the senescence phenotype. |
About TF
| TF name : | JUN(AP-1,AP1,c-Jun,cJUN,p39) | |
| TF experiment: | CRISPR/Cas9,Luciferase Reporter Assay | |
| TF experiment description: | We thus constructed a CRISPR-Cas9 sgRNA library designed to target senescence-induced Enhancers that are putatively regulated by AP-1 and used it in a functional screen.That screen was confined to regions that are directly bound by p53 as detected by p53 chromatin immunoprecipitation-sequencing (ChIP-seq) analysis and therefore missed Enhancers that are critical for OIS enforcement and are regulated by other TFs, in either a p53-dependent or -independent manner. |
About Function
| Enhancer function : | We identify FOXF1 as the gene regulated by this enhancer and demonstrate that FOXF1 mediates EnhAP1-OIS1 effect on the senescence phenotype. |
| Enhancer function experiment: | qRT-PCR,Western blot |
| Enhancer function experiment description: |
the physical interactions between EnhAP1-OIS1 and the promoter region of FOXF1 were significantly stronger , suggesting a robust transcriptional regulation of EnhAP1-OIS1. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
