About Enhancer
| Enhancer ID: | E_01_217 | |
| Enhancer symbol: | EBAs Enhancer | |
| Species: | Human | |
| Position : | chr2:98362566-98364423   |
|
| Biosample name: | HEK-293 | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 26216945 |
| Enhancer experiment: | ChIP-seq,Hi-C,Luciferase Reporter Assay | |
| Enhancer experiment description: | Similarly, we integrated topologically associated domain (TAD) boundaries inferred from Hi-C chromatin contact maps (34) and found that the MIR insulators are also closer to TAD boundaries (P < 5.4E-8, Mann–Whitney test) (Fig. 1 C and D).These findings suggest the predicted MIR insulators have domain barrier function. In addition, we used the ChIA–PET interaction data from human CD4+ T cells (35) to test whether the predicted MIR insulators can potentially block enhancer–promoter interactions. We focused on ChIA–PET interactions between enhancers and promoters that are proximal (<500 kb) to MIR-derived insulators and classified them into one-side interactions, i.e., the interaction’s anchors are restricted to one-side of a MIR insulator, and cross-interactions, i.e., the interaction’s anchors are separated by a MIR insulator (Fig. 1E).For the human EBA, a luciferase reporter construct transfected in human HEK 293 cells (Materials and Methods) was used to evaluate three predicted MIR insulators (SI Appendix, Table S2). All three MIR insulators tested here showed enhancer-blocking activity comparable to the 5′ HS4 positive control (Fig. 2A) and much larger insulator activity than the second positive control, i.e., the minimal insulator sequence motif of 5′ HS4 (II/III). |
About Target gene
| Target gene : | ZAP70(ADMIO2,IMD48,SRK,STCD,STD,TZK,ZAP-70) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay | |
| Target gene experiment description: | ll three MIR insulators tested here showed enhancer-blocking activity comparable to the 5′ HS4 positive control (Fig. 2A) and much larger insulator activity than the second positive control, i.e., the minimal insulator sequence motif of 5′ HS4 (II/III).The blocking of the repressive chromatin domain also appears to be functionally important to CD4+ T cells because one of the proximal genes in the adjacent active chromatin domain, i.e., gene ZAP70, is part of the T-cell receptor pathway and its promoter is involved with multiple active ChIA–PET interactions (Fig. 2D). |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
