About Enhancer
| Enhancer ID: | E_01_184 | |
| Enhancer symbol: | DHS-44 Enhancer | |
| Species: | Human | |
| Position : | chr7:117075514-117075933   |
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| Biosample name: | Human Bronchial Epithelial Cell | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 25259561 |
| Enhancer experiment: | Luciferase Reporter Assay,EMSA,RT-PCR,ChIP | |
| Enhancer experiment description: | To confirm that DHS-44kb contained an enhancer of CFTR expression, the 0.6-kb DHS fragment (hg 19, chr7:117075400–117076000) was PCR amplified and inserted into the enhancer site of the pGL3B 245 vector.These data suggest that the enhancer activity of the DHS-44kb element is specific to airway epithelial cells. |
About Target gene
| Target gene : | CFTR(ABC35,ABCC7,CF/MRP,MRP7,TNR-CFTR,dJ760C5.1\) | |
| Strong evidence: | -- | |
| Less strong evidence: | RT-PCR,ChIP | |
| Target gene experiment description: | To determine whether the SFN_x0002_triggered antioxidant response regulates CFTR expression directly through the DHS-44(279) cis-element, ChIP was used to measure the enrichment levels of the constitutive ARE binding factor Bach1, the SFN-induced ARE binding factor Nrf2, and their common partner MafK at this site. |
About TF
| TF name : | NFKB1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50)BACH1(BACH-1,BTBD24) | |
| TF experiment: | EMSA | |
| TF experiment description: | EMSAs were performed with 32P-labeled, double-stranded DNA probes for 44-I, 44-II, and 44-III,Moreover, when Bach1 was overexpressed in 16HBE14o- cells by transient transfection of a mouse Bach1 cDNA clone, a strong supershift was seen with the Bach1 antibody and probe 44-I. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
