About Enhancer
| Enhancer ID: | E_01_180 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr12:48368875-48369590   |
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| Biosample name: | 10T1/2,MC615 Cells,MCF-7 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Osteoarthritis | |
| DO: | DOID:8398 | |
| Mesh: | D010003 | |
| Distance from TSS: | Intron | |
| Pubmed ID: | 25081415 |
| Enhancer experiment: | RT-PCR,ChIP,Luciferase Reporter Assay,EMSA | |
| Enhancer experiment description: | Thus, several binding sites of the intronic enhancer have been shown to interact with transcription factors, such as Sox-9,Sox-6, and L-Sox-5, acting cooperatively and consequently activating expression of COL2A1 in 10T1/2 and MC615 cells.Therefore, in order to validate our in vitro hypoth_x0002_esis and to determine whether these transcription factors were effectively able to bind in vivo to Col2a1-specific enhancer,we performed ChIP assays in primary chondrocytes using primers mapping the +2,383/+2,611-bp region of COL2A1 first intron. This region includes a Sox-9 cis-element (+2,384/+2,404 bp), one HMG binding site (+2,388/+2,394 bp), and one Sp1/Sp3 binding sequence (+2,440/+2,485 bp) but does not harbor ERE cis-sequence. |
About Target gene
| Target gene : | COL2A1(ANFH,AOM,COL11A3,SEDC,STL1) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP,EMSA | |
| Target gene experiment description: | Altogether, these EMSA assays indicate that 17β-E2 and hERα66 enhance transcription of COL2A1 by increasing the binding activity of Sp1 and to a lesser extent of Sp3, not only to COL2A1 promoter but also to COL2A1-specific enhancer. |
About TF
| TF name : | SP1(SP1)SP3(SPR2)SOX9(CMD1,CMPD1,SRA1,SRXX2,SRXY10)SOX6(HSSOX6,SOXD) | |
| TF experiment: | ChIP,RT-PCR,EMSA | |
| TF experiment description: | To identify what protein partners could be involved in the ERα66 recruitment, we performed ReChIP experiments using an anti-ERα antibody. Our data showed that ERα66 was bound indirectly to Col2a1 Enhancer through protein–protein interactions with Sp1, Sp3, and Sox-9 transcription factors in primary as well as dedifferentiated articular chondrocytes. |
About Function
| Enhancer function : | Several binding sites of the intronic Enhancer have been shown to interact with transcription factors, such as Sox-9,Sox-6,and L-Sox-5, acting cooperatively and consequently activating expression of COL2A1 in 10T1/2 and MC615 cells. |
| Enhancer function experiment: | ChIP,EMSA |
| Enhancer function experiment description: |
By contrast to transient transfection data obtained with reporter construct pGL2-3.316 kb, these last results suggest that Sox-9/Sox-6/L-Sox-5, as well as the COL2A1 enhancer region, might also participate in the anabolic effect of 17β-E2 on COL2A1 in primary and dedifferentiated chondrocytes since the hormone significantly increases their DNA-binding activity to this region. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
