About Enhancer
| Enhancer ID: | E_01_173 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr8:143896217-143898217   |
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| Biosample name: | Human Diploid BJ Fibroblast Cell Lines | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 24797517 |
| Enhancer experiment: | ChIP-qPCR,ChIP,ChIP-seq | |
| Enhancer experiment description: | On the other hand,only 4% are positive for H3K4me3, indicating that these sites are likely to represent regulatory enhancer elements.Furthermore, we validated by ChIP-qPCR that p53 and JMJD3 display an IR responsive recruitment to several putative enhancer elements.We confirmed that these sites possess features of active enhancers; they are DNase I positive，show binding of p300 and have enrichment of H3K4me1 and H3K27ac but not H3K4me3 and H3K27me3.We also observed that exposure to IR correlated with an increased binding of p300 and subsequently acetylation of H3K27,which is in agreement with data demonstrating that p53 interacts with p300. |
About Target gene
| Target gene : | GML(LY6DL) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP-qPCR | |
| Target gene experiment description: | Examples of p53 (before and after IR treatment) and JMJD3 (before and after IR treatment), H3K4me3 and DNase I-seq tracks at two putative enhancer elements located 11 kb upstream of CDKN1A (upper panel) or 19 kb upstream of GML (lower panel).Corresponding ChIP-qPCR validations of the binding of p53, JMJD3 and p300 as well as the levels of histone modifications H3K4me1, H3K4me3, H3K27ac and H3K27me3 at the two distal binding sites listed above. |
About TF
| TF name : | TP53(BCC7,BMFS5,LFS1,P53,TRP53) | |
| TF experiment: | ChIP-seq | |
| TF experiment description: | To understand if JMJD3 and p53 also display genomic co_x0002_localization, we performed global mapping of JMJD3 and p53 binding sites in telomerase-immortalized human BJ diploid fibroblasts by chromatin immunoprecipitation followed by se_x0002_quencing (ChIP-seq). |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
