About Enhancer
| Enhancer ID: | E_01_144 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr21:43771491-43773491   |
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| Biosample name: | MCF-7,MDA-MB-231,293T | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Mixed-Lineage Leukemia 1 | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 24288367 |
| Enhancer experiment: | qRT-PCR,ChIP | |
| Enhancer experiment description: | TFF1 has three EREs, and ERE3 at -9.9 kb upstream of transcriptional start site (TSS) is the major ER binding site and considered to be the major enhancer element. Our chromatin immunoprecipitation (ChIP) assay showed that the enhancer region of the TFF1 gene (ERE3,-9.9 kb) was highly occupied by MLL1 and that the recruitment of MLL1 to this region was further enhanced after E2 treatment in MCF-7 cells (Figure 2A).We validated these findings by a ChIP scanning assay,which surveyed from _x0002_10 kb upstream through 5 kb downstream of the TSS of the TFF1 gene. The distal enhancer region of TFF1 was found to be highly occupied by MLL1. |
About Target gene
| Target gene : | TFF1(BCEI,D21S21,HP1.A,HPS2,pNR-2,pS2) | |
| Strong evidence: | -- | |
| Less strong evidence: | qRT-PCR,ChIP | |
| Target gene experiment description: | When FLAG-tagged MLL1-CX or MLL1-CXPB was over_x0002_expressed in MCF-7 cells by transient transfection, quan_x0002_titative ChIP assays performed with anti-FLAG antibody demonstrated the occupancy by both MLL1-CX and MLL1-CXPB on ERE3 (but not ERE2 or ERE1) of the TFF1 gene, indicating that the 40 kDa MLL1-CX fragment is sufficient for recruitment to the enhancer region in vivo (Figure 3F) with the same genomic binding specificity as wild-type MLL3. |
About TF
| TF name : | KMT2A(KMT2A) | |
| TF experiment: | qPCR,ChIP | |
| TF experiment description: | FLAG-tagged MLL1-CX or MLL1-CXPB was transiently expressed in the MCF-7 cells, which were then grown in hormone-free media for 48 h and then treated with 100nM E2 for 1 h before performing the ChIP assay with anti-FLAG antibody. Precipitated DNA was analyzed by qPCR with primers representing the ER binding sites of the TFF1 genes. |
About Function
| Enhancer function : | Mixed-lineage leukemia 1 (MLL1) protein is a key determinant that maintains permissive chromatin structure of the TFF1 Enhancer region. |
| Enhancer function experiment: | ChIP,NOMe-seq |
| Enhancer function experiment description: |
Mixed-lineage leukemia 1 (MLL1) protein is a key determinant that maintains permissive chromatin structure of the TFF1 enhancer region. MLL1 occupies the TFF1 enhancer region and methylates H3K4 before hormone stimulation. In vitro, MLL1 binds directly to the CpG-rich region of the TFF1 enhancer, and its binding is dependent on hypomethylation of DNA. Furthermore, the depletion of MLL1 in MCF-7 cells results in a dramatic decrease of chromatin accessibility and recruitment of FOXA1 and ERa to the enhancer element. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
