About Enhancer
| Enhancer ID: | E_01_119 | |
| Enhancer symbol: | P53BER4 Enhancer | |
| Species: | Human | |
| Position : | chr1:181104024-181104549   |
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| Biosample name: | MCF-7 | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 23273978 |
| Enhancer experiment: | qRT-PCR,4C,ChIP-seq,Luciferase Reporter Assay,ChIP | |
| Enhancer experiment description: | Interestingly, many of these regions possess conserved p53-binding sites and all known hallmarks of enhancer regions. We demonstrate that these p53-bound enhancer regions (p53BERs) indeed contain enhancer activity and interact intra_x0002_chromosomally with multiple neighboring genes to convey long-distance p53-dependent transcription regulation. |
About Target gene
| Target gene : | MDM2(ACTFS,HDMX,hdm2) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP | |
| Target gene experiment description: | To identify possible target genes of p53-dependent enhancer domains, we applied chromosome conformation capture (4C) technology in combination with next-generation sequencing.). To confirm that these genes are indeed regulated by p53, we treated cells with the MDM2-inhibitor Nut_x0002_lin-3 and analyzed mRNA levels of one prominent neighboring gene for each p53BER. |
About TF
| TF name : | TP53(BCC7,BMFS5,LFS1,P53,TRP53) | |
| TF experiment: | ChIP | |
| TF experiment description: | Importantly, the enhancer activity of all p53BERs studied here was p53 dependent, as cotransfection of a p53 knockdown vector (p53KD) significantly inhibited enhancer activity. |
About Function
| Enhancer function : | p53BERs produce enhancer RNAs (eRNAs) that are required for efficient transcriptional enhancement of interacting target genes and induction of a p53-dependent cell-cycle arres |
| Enhancer function experiment: | qRT-PCR,4C,ChIP |
| Enhancer function experiment description: |
We show that eRNAs are functional ncRNAs involved in transcription enhancement of genes interacting with the Enhancer they are produced from. Thus, our results imply that p53 can regulate transcription of multiple distantly located genes through binding to Enhancers and that eRNAs produced from these Enhancers are involved in transcrip_x0002_tion enhancement. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
