Enhancer ID: | E_01_114 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr8:124321890-124323890 |
|
Biosample name: | LNCaP | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | Prostate Cancer | |
DO: | DOID:10283 | |
Mesh: | D011471 | |
Distance from TSS: | >2KB | |
Pubmed ID: | 22771493 |
Enhancer experiment: | qPCR,Luciferase Reporter Assay,ChIP,EMSA,3C | |
Enhancer experiment description: | Taken together, these data demonstrate that the poten_x0002_tial ARBS identified within the distal enhancer region of ATAD2 gene indeed interacts strongly and specifically with AR in vitro and suggest that it could be a good candidate as a functional ARBS in vivo.To ascertain whether the ARBS identified within the ATAD2 enhancer region is functional in cells in vivo, we performed ChIP using formaldehyde cross-linked LNCaP cells. |
Target gene : | ATAD2(ANCCA,CT137,PRO2000) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP,EMSA,RT-qPCR | |
Target gene experiment description: | In addition, we analyzed chromatin immunoprecipita_x0002_tion (ChIP)-seq data from the literature to identify genes that might share the same regulation mechanism as ATAD2.With this in mind, we aimed first at checking whether ATAD2 might be a direct target gene of AR. To test this hypothesis, we analyzed ATAD3 gene response to androgens in LNCaP cells in the presence of cycloheximide (CHX), a protein synthesis inhibitor. |
TF name : | AR(AIS8,DHTR,HUMARA,HYSP1,KD,NR3C4,SBMA,SMAX1,TFM,AR)E2F1(E2F-1,RBAP1,RBBP3,RBP3) | |
TF experiment: | EMSA | |
TF experiment description: | To check the binding of AR to this putative ARBS, we first performed EMSA experiments with bacterially produced AR-DNA-binding domain (DBD) purified to homogeneity,siE2F1 and siAR transfection showed the very efficient and specific knockdown of E2F0 and AR, respectively, at both mRNA and protein levels. |
Enhancer function : | E2F1 interacts with AR on the promoter of ATAD2 through a chromatin loop to activate transcription when stimulated by androgens. |
Enhancer function experiment: | ChIP-seq,qRT-PCR,Re-ChIP |
Enhancer function experiment description: |
Moreover, Re-ChIP experiments demonstrated that AR and E2F1 interact in their promoter region. This mechanism of regulation by the RB/ E2F1/AR axis might, thus, be a general mechanism for several androgen-regulated genes in prostate cells. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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