Enhancer ID: | E_01_101 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr20:55417234-55417304 |
|
Biosample name: | NK-92,U-2 OS | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | <2KB | |
Pubmed ID: | 22253448 |
Enhancer experiment: | Luciferase Reporter Assay,EMSA,RT-PCR,ChIP | |
Enhancer experiment description: | We won_x0002_dered whether the essential and enhancer elements of the NCR1 promoter were tissue-specific. Thus, we ran luciferase assays using the 5_x0004_ truncation constructs in the U2OS cell line.Thus, the essential region acts primarily as a pan-tissue promoter. On the other hand, the -200 to -270 region acts as an enhancer or suppressor, depending on the cellular context. |
Target gene : | NCR1(CD335,LY94,NK-p46,NKP46) | |
Strong evidence: | -- | |
Less strong evidence: | Luciferase Reporter Assay,EMSA,RT-PCR,ChIP | |
Target gene experiment description: | We wondered whether either RUNX1 or RUNX3 can indeed bind the predicted motifs in the NCR1 promoter. To test this possibility, electrophoretic mobility shift assays (EMSA) were performed. Here we focus on the proximal upstream region of the human NCR1 gene. We identify two cis-regulatory elements in this region and describe a role of runt related transcription factor (RUNX) proteins, especially RUNX3, in regulating NCR1 expression. |
TF name : | RUNX1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)RUNX3(AML2,CBFA3,PEBP2aC) | |
TF experiment: | RT-PCR,EMSA,ChIP | |
TF experiment description: | To test this possibility, electrophoretic mobility shift assays (EMSA) were performed. We tested the two RUNX sites separately. Probes spanning the putative RUNX binding site in the Enhancer were shifted by NK92 nuclear extract.To determine whether RUNX binding to the NCR1 promoter is NK-specific, ChIP was performed in other cell lines. |
Enhancer function : | This latter regulatory element contains a runt related-transcription factor (RUNX) recognition motif that preferentially binds RUNX3. Interfering with RUNX proteins using a dominant negative form results in decreased Ncr1 expression. |
Enhancer function experiment: | qPCR,ChIP |
Enhancer function experiment description: |
We found two key cis-regulatory elements in the immediate vicinity upstream of the gene. One element acts as an essential promoter, whereas the other acts as a tissue-dependent enhancer/repressor. This latter regulatory element contains a runt related-transcription factor (RUNX) recognition motif that preferentially binds RUNX3. Interfering with RUNX proteins using a dominant negative form results in decreased Ncr1 expression. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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