About Enhancer
| Enhancer ID: | E_01_084 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr17:32565596-32566796   |
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| Biosample name: | U-87MG | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 21551367 |
| Enhancer experiment: | Luciferase Reporter Assay,PCR,ChIP,EMSA,3C | |
| Enhancer experiment description: | Schematic of CCL2 locus showing regions that were tested in the reporter assays. |
About Target gene
| Target gene : | CCL2(GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF) | |
| Strong evidence: | 3C | |
| Less strong evidence: | PCR,qPCR | |
| Target gene experiment description: | The –16.5 ECR physically interacts with CCL2 proximal promoter.To detect such physical interactions we used the 3C assay. In the 3C assay, interaction between widely separated regulatory elements is detected. |
About TF
| TF name : | NFKB1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50)SP1(SP1)CEBPB(C/EBP-beta,IL6DBP,NF-IL6,TCF5) | |
| TF experiment: | ChIP,EMSA | |
| TF experiment description: | The EMSA and the pulldown experiments demonstrated in vitro interaction of the –16.5 ECR with NF-kB and c/EBPb. We used ChIP assay to determine whether the –16.5 ECR can bind NF-kB and c/EBPb in vivo. Sheared chromatin that was obtained from U87MG cells (treated or not treated with TNF-a) was immmu_x0002_noprecipitated with Abs directed against p65 and p50 subunits of NF-kB and c/EBPb or with control Abs. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
