Enhancer ID: | E_01_052 | |
Enhancer symbol: | WT1 Enhancer | |
Species: | Human | |
Position : | chr11:32403489-32405489 |
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Biosample name: | Human Podocyte Cell Line AB8/13 | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 21378152 |
Enhancer experiment: | qRT-PCR,Luciferase Reporter Assay | |
Enhancer experiment description: | We tested the effect of TGF-beta1 on a human WT1 enhancer located ∼4.3 kb upstream of the transcription start site using a reporter vector WTA. |
Target gene : | WT1(AWT1,GUD,NPHS4,WAGR,WIT-2,WT33) | |
Strong evidence: | -- | |
Less strong evidence: | qRT-PCR,Luciferase Reporter Assay | |
Target gene experiment description: | TGF-beta1 did not alter luciferase activity of the reporter construct for a human WT1 promoter but reduced that for a human WT1 5′ enhancer construct, suggesting that TGF-beta1 may regulate WT1 expression by altering the 5′ enhancer activity. |
TF name : | SMAD4(DPC4,JIP,MADH4,MYHRS) | |
TF experiment: | Luciferase Reporter Assay | |
TF experiment description: | The luciferase activity of p3TP-lux, a reporter construct for Smadbinding element, was determined after incubation with 5 ng/mL TGFbeta1 for 24 h. TGF-beta1 increased the luciferase activity by ~12-fold in the presence of the NTC shRNA, while this increase was largely prevented by Sh-Smad4. The luciferase activity of p3TP-lux was normalized to beta-galactosidase activity of pCMV-beta-gal and shown as relative values to that of pGL2 basic. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
-- |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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